| 四藤方对Bel-7402细胞增殖和凋亡作用研究 |
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| 引用本文： | 沈克平,胡兵,刘威.四藤方对Bel-7402细胞增殖和凋亡作用研究[J].中国实验方剂学杂志,2011,17(3):120-123. |
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| 作者姓名： | 沈克平 胡兵 刘威 |
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| 作者单位： | 上海中医药大学附属龙华医院肿瘤五科,上海,200032 |
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| 基金项目： | 国家自然科学基金(30873258); 上海市基础研究重点项目(09JC1413600); 上海市浦东新区中医领军型人才项目(PWZ2008-20-104); 上海市级医院临床科研资源共享平台(SHDC12007206) |
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| 摘 要： | 目的:观察清热解毒中药-四藤方对肝癌细胞Bel-7402增殖和凋亡的影响。方法:不同浓度四藤方处理人肝癌细胞Bel-7402、人肝细胞HL-7702,WST-8法检测细胞增殖,流式细胞术检测细胞凋亡,Hoechst 33258染色检测细胞凋亡形态变化,Western Blot法检测半胱天冬酶-3(caspase-3)活化。结果:与对照组比较,四藤方对人肝细胞HL-7702增殖无明显影响;100～400μg.mL-1四藤方作用24 h可显著抑制肝癌Bel-7402细胞增殖,促使细胞Bel-7402凋亡,呈现细胞凋亡形态,并可活化Bel-7402细胞caspase-3。结论:四藤方可以抑制Bel-7402细胞增殖,促Bel-7402细胞凋亡,并可能与活化caspase-3相关。
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| 关 键 词： | 肝癌细胞 四藤方 增殖 凋亡 半胱天冬酶-3 |
| 收稿时间： | 2010/8/23 0:00:00 |
Effects of Siteng Fang on Proliferation and Apoptosis in Bel-7402 Cells in vitro |
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| SHEN Ke-ping,HU Bing and LIU Wei.Effects of Siteng Fang on Proliferation and Apoptosis in Bel-7402 Cells in vitro[J].China Journal of Experimental Traditional Medical Formulae,2011,17(3):120-123. |
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| Authors: | SHEN Ke-ping HU Bing and LIU Wei |
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| Institution: | SHEN Ke-ping,HU Bing,LIU Wei(The 5th Department of Oncology,Longhua Hospital,Shanghai University of Traditional ChineseMedicine,Shanghai,20032,China) |
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| Abstract: | Objective:To observe the effects of Siteng Fang(STF),a Chinese herb formula,on proliferation and apoptosis in hepatocarcinoma Bel-7402 cells in vitro.Method: Human hepatocarcinoma Bel-7402 cells and human normal liver HL-7702 cells were treated with different dose of STF.Cell proliferation was detected with CCK-8(cell counting kit-8,CCK-8) assay;cell apoptosis was detected by flow cytometry.Apoptotic morphology was visualized by hoechst 33258 staining,and activation of caspase-3 was detected by western blot... |
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| Keywords: | hepatocarcinoma cell Siteng Fang proliferation apoptosis caspases-3 |
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