大鼠颅骨体外培养矢状缝牵引成骨模型的建立

目的探讨建直幼年SD大鼠顿骨器官体外培养矢状缝牵引成骨模型。方法取19日龄的SD大鼠颅顶骨矢状缝组织块．随机分为对照组和实验组进行体外器官培养。实验组加力约3．92×10^3N（0．4g）力Hr照组不加力。培养24h结束时，进行大体观察及倒置显微镜下观察；并经苏木精-伊红染色后进行组织学观察。结果大体观察／受倒置显微镜下观察发现，实验组骨缝逐渐明显加宽。至加力24h，所有标本欠状缝未见断裂。组织学观察发现，缝两侧区域为成骨活跃部位，两侧骨化前沿交错排列。缝内可见纤维结缔纠织、成骨细胞、成纤维细胞及毛细血管，缝内组织与硬脑膜相连。对照组骨缝保持正常发育，未见明显变化，以成骨细胞为主。结论大鼠颅骨骨缝器官可以在体外培养中成功存活并继续生长。

Rat cranial sutures in vitro: a new experimental model for studying the osteogenic effect to tensile force

Objective To develop an in vitro model for cranial suture of neonatal SD rat. Methods The parietal hones with the sagittal suture were removed from SD rats (19-days old) for organ culture. In the experimental group, tensile force 3.92×10~(-3) N (0.4 g) was applied by helical springs, whereas no tension (0 N) was set in control group. These explants were observed under inverted microscope. At the end of the incubation period for 24 hours, general conditions were observed under inverted microscope and histological conditions were observed after hematoxylin and eosin stain. Results Under inverted micro-scope, sutures had no obvious changes in control group, whereas sutures were enlarged gradually in the experimental group. With histological observation, sutures developed normally in control group, but in experimental group, osteohlasts and capillary vessels proliferated actively in the suture. Conclusions In vitro model of cranial suture can be cultured and grown successively.