| 曲美他嗪对大鼠心肌缺血-再灌注损伤的保护作用 |
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| 引用本文： | 颜永进,张跃明,陆洋,王世亚.曲美他嗪对大鼠心肌缺血-再灌注损伤的保护作用[J].江苏医药,2011,37(1). |
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| 作者姓名： | 颜永进 张跃明 陆洋 王世亚 |
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| 作者单位： | 南通大学附属海安医院心脏病科,江苏省海安县,226600 |
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| 摘 要： | 目的探讨曲美他嗪(TMZ)对缺血-再灌注(I-R)后大鼠心肌细胞凋亡的保护作用。方法 SD大鼠随机分为假手术组(A组)I、-R组(B组)、低浓度TMZ(1 ml/100 g)组(C组)和高浓度TMZ(3 ml/100 g)组(D组),每组6只。制备大鼠心肌I-R模型,采用流式细胞仪(FCM)、原位末端标记(TUNEL)技术、免疫组化检测心肌组织细胞凋亡情况,并观察细胞形态变化。结果 B组细胞凋亡指数、TUNEL阳性细胞数、Bcl-2、Bax较A组增高(0.2416±0.0263)vs.(0.0469±0.0136),(3.213±0.14)vs.(0.44±0.133),(2.057±0.187)vs.(0.47±0.082),(4.257±0.135)vs.(0.52±0.058)(P<0.05)],而C、D组细胞凋亡指数、TUNEL阳性细胞数、Bax明显下降(P<0.05),D组减低更明显。结论 TMZ可抑制心肌I-R损伤中的细胞凋亡。
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| 关 键 词： | 心肌缺血-再灌注损伤 曲美他嗪 |
The protective effect of trimetazidine on the myocardial ischemia-reperfusion injury in rats |
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| Abstract: | Objective To investigate the protective effect of trimethazine(TMZ) on rat myocardial cell apoptosis after ischemia-reperfusion(IR).Methods Twenty-four SD rats were randomly and equally divided into 4 groups of A(sham operated),B(I-R model),C(treated with TMZ 1ml/100g) and D(treated with TMZ 3ml/100g).The myocardial I-R model in rats was established.The myocardial cell apoptosis was respectively detected by flow cytometry(FCM),TUNEL technology and immunohistochemistry,and the changes of cell morphology were... |
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| Keywords: | Myocardial ischemia-reperfusion injury Trimetazidine |
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