中国北方汉族男性血管内皮生长因子受体2基因+4422(AC)n多态性与HiHiLo训练敏感性的关联研究

目的:探讨中国北方汉族男性血管内皮生长因子受体2(VEGFR2)基因+4422(AC)n多态性与高住高练低训(HiHiLo)训练敏感性的关联。方法:选取71名中国北方平原地区汉族男子进行30天HiHiLo,方案为每日在低氧房(O2浓度为14.8%～14.3%,模拟海拔2800～3000米)居住10小时,每周进行3次75%VO2max强度的低氧训练(O2浓度为15.4%～14.8%,模拟海拔2500～2800米),运动时间为30 min/次,其余时间在常氧环境下训练。在HiHiLo前、后测定VO2 max和SpO2。其中SpO2的测定采用低氧环境下(15.4%O2,模拟海拔约2500米)的定量负荷运动实验,运动强度为HiHiLo前个体75%VO2max,运动时间15min。基因分型采用PCR结合荧光标记复合STR-genescan方法检测+4422(AC)n多态重复次数。结果:HiHiLo后,VO2 max以及定量负荷运动中SpO2均显著性提高,且(AC)11/(AC)11基因型者rVO2max的训练敏感性显著高于(AC)11/(AC)12基因型者。结论:VEGFR2基因+4422(AC)n多态性与HiHiLo后rVO2 max训练敏感性有关联,而与定量负荷下SpO2训练敏感性无关联。提示+4422(AC)n多态性可以作为预测低氧训练效果的分子遗传学标记,但还需加大样本量进一步验证。

Association of VEGFR2 Gene+4422(AC)n Polymorphism with the Sensitivity to HiHiLo Training in Men of Han Nationality

Objective The purpose of this study was to explore the association between VEGFR2 gene+4422(AC)n polymorphism to the sensitivity of HiHiLo.Methods Seventy one men of Han nationality in northern China underwent HiHiLo for 30 days.The subjects stayed in the hypoxic environment(14.8%～14.3%O2)10 hours per day,and performed 30-minute hypoxic exercise(15.4%～14.8%O2) three times per week at the intensity of individual baseline VO2 max.The remaining training courses were completed at sea level.VO2 max and SpO2 were monitored before and after HiHiLo,in which SpO2 was tested through fixed load exercise in hypoxic environment(15.4%O2)at the intensity of individual baseline VO2 max.The genotypes were analyzed by STR-genescan.Results The absolute(L·min-1)and relative(ml·kg-1·min-1)VO2max and SpO2 during fixed load exercise significantly increased after hypoxic training.The rVO2max of the(AC)11/(AC)11 genotype was significantly sensitive to HiHiLo as compared with the(AC)11/(AC)12.Conclusion There was an association between VEGFR2 gene +4422(AC)n polymorphism and the sensitivity of rVO2max to HiHiLo,while the polymorphism was not associated with the sensitivity of SpO2 during fixed load exercise to HiHiLo.It was suggested that VEGFR2 gene +4422(AC)n polymorphism could be used as a genetic marker for estimating the response to hypoxic training.Further investigation with greater sample size is needed to validate this finding.