慢性阻塞性肺疾病患者对氧磷酶和氧化应激与全身炎性反应的研究

Objective To analyze the activity of paraoxonase (PON1) and explore the relationship of PON1 and oxidative stress with systemic inflammation response in the acute exacerbation phase and stationary phase in patients with chronic obstructive pulmonary disease (COPD). Methods Serum PON1 activity was measured by phenylacetate in 38 patients with COPD and 30 healthy people. The activity of glutathione peroxidase (GSH-Px) was detected by improved Hafeman method. Total antioxidant capacity (TAC) was measured by eolorimetry and malondialdehyde (MDA) level was measured by thiobarbituric acid colouration method. The interleukin-6 (IL-6) and interleukin-8 (IL-8) levels were detected by radioimmunoassay. The level of C-reactive protein (CRP) was measured by immune turbidimetry. Results In the acute exacerbation phase, the activity of serum PON1 was significantly lower in COPD group than in control group [(98.03±42.40)×103U/L vs. (136.00±60. 50)×103U/L, t=4.962, P<0.01], and it was negatively related to the IL-8 level (r= - 0. 589, P<0.01) and positively related to FEV1% (r= 0. 434, P<0. 05). The activity of GSH-Px was negatively related to the IL-6 level (r=-0. 362, P< 0. 05). In the stationary phase of COPD group, the activity of serum PON1 had no statistical difference compared with control group[(131.50±53.65))×103U/L vs. (136. 00±60.50)×103U/ L, t=2. 457, P>0. 053, and it was negatively related to the IL-8 level (r=-0. 563, P<0.05). Conclusions Serum PON1 activity is significantly decreased in acute exacerbation phase of COPD group compared with control group and it is positively related to FEV1%. The oxidative stress is closely related to systemic inflammation response in patients with acute exacerbation of COPD.

Relationship of serum paraoxonase and oxidative stress with systemic inflammatory response in patients with chronic obstructive pulmonary disease