| 新生大鼠神经干细胞的体外分离和培养 |
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| 引用本文： | 龚珊 单立冬 郭试瑜 久光正 蒋星红. 新生大鼠神经干细胞的体外分离和培养[J]. 苏州大学学报(自然科学版), 2005, 25(1): 71-73 |
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| 作者姓名： | 龚珊 单立冬 郭试瑜 久光正 蒋星红 |
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| 作者单位： | [1]苏州大学医学院神经生物学教研室，江苏苏州215007 [2]日本昭和大学医学部第一生理学教室，日本东京 [3]苏州大学医学院神经生物学教研室，江苏苏州215007／／日本昭和大学医学部第一生理学教室，日本东京 |
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| 基金项目： | 苏州大学医学发展基金资助(No.EEl34033)中日校际合作基金资助(No EE134005) |
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| 摘 要： | 目的改进大鼠神经干细胞的分离和培养方法。方法用出生1～3d的新生大鼠，取脑用刀片剁碎和吸管吹打的机械分离法代替酶消化法。用免疫细胞化学法鉴定神经干细胞、神经元和星形胶质细胞。结果用机械分离法从新生大鼠脑组织分离神经干细胞的获得率较高，并培养出较多的神经球，还能分化成神经元和星形胶质细胞。结论该方法简便，能得到较多的神经球，细胞获得率也较高。
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| 关 键 词： | 新生大鼠 神经干细胞 细胞培养 |
| 文章编号： | 1673-0399(2005)01-0071-03 |
| 修稿时间： | 2004-06-18 |
Isolation and Culture of Neural Stem Cells from Neonatal Rat Brain in Vitro |
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| GONG Shan,SHAN Li-dong,JIANG Xing-hong,et al. Isolation and Culture of Neural Stem Cells from Neonatal Rat Brain in Vitro[J]. Suzhou University Journal of Medical Science, 2005, 25(1): 71-73 |
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| Authors: | GONG Shan SHAN Li-dong JIANG Xing-hong et al |
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| Abstract: | Objective To modify the isolation and culture method of neural stem cells from rats. Methods Neonatal (1 ~ 3 d after birth ) rats were used . The neural stem cells were isolated with mechanical dispersion method in stead of enzymatic digestion method. The immunocytochemistry was used to identify the neural stem cells, neurons and astrocytes. Results More neurospheres and high cell harvest rate were obtained from neonatal rat brain with mechanical dispersion method. The isolated cells could differentiate to neurons and astrocytes. Conclusion This isolation and culture method is simple, the number of neurospheres is more and the cell havest rate is higher. |
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| Keywords: | neonatal rat neural stem cell cell culture |
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