| 丙肝核酸疫苗免疫的CTL活性检测 |
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| 引用本文： | 尤崇革,李益民,白植生.丙肝核酸疫苗免疫的CTL活性检测[J].中国免疫学杂志,2002,18(6):392-393. |
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| 作者姓名： | 尤崇革 李益民 白植生 |
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| 作者单位： | 1. 兰州医学院第二附属医院,兰州,730030
2. 兰州生物制品研究所,兰州,730030 |
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| 摘 要： | 目的 :探讨丙肝核酸疫苗的细胞免疫应答效果。方法 :用丙肝核酸疫苗pSVL HCV C E1 通过皮下注射途径免疫BALB C小鼠 ,以绿色荧光载体重组体pEGFP N1 HCV C E1 转染的小鼠骨髓瘤细胞SP2 0作为靶细胞 ,采用51 Cr释放法 ,以(Na) 2 51 CrO4 标记靶细胞进行标准的 4h杀伤试验检测其CTL活性。结果 :CTL杀伤率达 4 0 7%。结论 :丙肝核酸疫苗能激发强烈的细胞免疫
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| 关 键 词： | 丙肝核酸疫苗 细胞免疫 CTL活性检测 |
| 文章编号： | 1000-484X(2002)06-0392-02 |
CTL activity detection of immune response to hepatitis C viral nucleic acid vaccine |
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| YOU Chong Ge,LI Yi Min,BAI Zhi Sheng.CTL activity detection of immune response to hepatitis C viral nucleic acid vaccine[J].Chinese Journal of Immunology,2002,18(6):392-393. |
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| Authors: | YOU Chong Ge LI Yi Min BAI Zhi Sheng |
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| Institution: | YOU Chong Ge,LI Yi Min,BAI Zhi Sheng.The Second Affiliated Hospital of Lanzhou Medical College,Lanzhou Institute of Biological Products,Lanzhou 730030 |
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| Abstract: | Objective:To discuss mice cellular immune response to the hepatitis C viral nucleic acid vaccine.Methods:Hepatitis C viral nucleic acid vaccine pSVL HCV/C E 1 was inoculated BALB/C mice by subcutaneous injection.To detect the CTL activity of immune mice,Made the standard 4 hours lysis test by labeling the target cells with (Na) 2 51 CrO 4,which was prepared from the syngentic BALB/C mouse myeloma derived cell strain SP2/0 transfected by the eukaryotic expression recombinant plasmid pEGFP N 1 HCV/C E 1.Results:The kill ratio of CTL is up to 40.7%.Conclusion:The result suggested hepatitis C viral nucleic acid vaccine could induce strong cellular immune response |
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| Keywords: | Hepatitis C viral nucleic acid vaccine Cellular immunization CTL activity detection |
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