| 流式细胞血小板交叉配型试验方法的建立 |
| |
| 引用本文： | 王嘉励,邓晶,徐秀章,丁浩强,陈扬凯,邵媛,叶欣. 流式细胞血小板交叉配型试验方法的建立[J]. 中国输血杂志, 2011, 24(6): 478-481 |
| |
| 作者姓名： | 王嘉励 邓晶 徐秀章 丁浩强 陈扬凯 邵媛 叶欣 |
| |
| 作者单位： | 广州血液中心 输血研究所,广东 广州,510095 |
| |
| 基金项目： | 广东省医学科研基金，广州市医药卫生科技项目 |
| |
| 摘 要： | 目的建立检测灵敏度更高的流式细胞血小板交叉配型(Flow-XM)的试验方法。方法对104名临床血小板输注>3次的患者作Flow-XM试验,同时采用经典补体依赖微量淋巴细胞毒交叉配型(NIH-CDC)方法作对照,比较2种方法的试验结果。结果血小板阳性检出率,Flow-XM试验为60.58%(63/104),NIH-CDC试验为26.92%(28/104)(P<0.01)。结论 Flow-XM试验具有较高的灵敏度,能够对原始结果全程质控,可使血小板交叉配型技术实现了标准化。
|
| 关 键 词： | 交叉配型 相关抗体 血小板 流式细胞术 HLA抗体 灵敏度 输注无效,血小板 |
Establishment of the experiment method for flow cytometry of platelet cross match |
| |
| Affiliation: | WANG Jiali,DENG Jing,XU Xiuzhang,et al.Institute of Blood Transfusion,Guangzhou Blood Center,Guangzhou 510095,China |
| |
| Abstract: | Objective To set up a more sensitive detection method of platelet cross match using flow-cytomerty.Methods Blood samples from 104 patients who had received platelet transfusion more than three times underwent Flow-XM and NIH-CDC detection.The results between two different techniques were compared.Results In 104 patients,63 were positive in Flow-XM(60.58%),28 were positive in NIH-CDC(26.92%),there was significant difference between two methods(P<0.01) Conclusion Flow-XM is more sensitive than NIH-CDC,additionally,the computer printouts represent a permanent record of the cross match for retrospective review.Flow-XM may become the standard cross match method as a possible alternative to be recommended. |
| |
| Keywords: | Cross-match Platelet Flow cytometry HLA antibody Sensitivity Transfusion refractory |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
