实验性龈炎的细菌成分变化的分析

目的:观察实验性龈炎发展过程中细菌成分的变化,分析细菌成分与临床指标间的关系。方法:选取11名受试者,在其停止口腔卫生措施后21d内(第0、2、4、7、14、21天)和恢复口腔卫生措施1周后(第28天)取龈下菌斑作刚果红涂片,分析细菌成分;同时记录临床指标的变化。结果:在实验性龈炎发展过程中,螺旋体的百分含量在基线时最低,随着停止刷牙的时间的延长而逐渐增加,并达到峰值,与基线存在显著性差异(P<0.05),恢复刷牙后迅速降低。其百分含量与各临床指标(菌斑指数、出血指数、牙龈指数)有显著性正相关(P<0.01),杆菌和球菌所占比例在试验过程中无明显变化(P>0.05)。结论:螺旋体的百分含量在基线、停止刷牙和恢复刷牙1周后存在显著差异,并与各临床指标有显著性正相关,其变化与牙龈炎症的形成和发展过程一致。

Changes of plaque microflora during experimental gingivitis

Objective:To observe the changes of plaque microflora during the experimental gingivitis and to analyze the relationship between the plaque microflora and the clinical parameters.Methods:11 young male subjects with healthy gingiva and without systemic disease were selected.Subgingival plaque samples(2 sites /person)were collected and then smeared by Gongo red at baseline(0 day),the 7th,14th,21st day(without oral hygiene)and 28th day(7 days after reestablishing oral hygiene)respectively during experimental gingivitis.At the same time the clinical parameters were recorded.The results of smear and the clinical parameters were analyzed.Results:The percentage of spirochete was the lowest at the baseline and increased during the experimental gingivitis,and then reached the highest level on the 21st day.The percentage of spirochete of the 21st day showed the significant different compared with that of baseline(P<0.05).After reestablishing oral hygiene it reduced to the level of the baseline.The percentage of spirochete showed the positive correlation with the clinical parameters,including plaque index(PLI),bleeding index(BI)and gingival index(GI)(P<0.01).The persentage of cocci and rod in plague were not significantly changed during the experiment(P>0.05)Conclusion:Spirochete is correlated to the development of the gingivitis.