别嘌呤醇对附子在两种模型中的影响及机制探讨

目的：研究别嘌呤醇对附子在腺嘌呤所致慢性肾衰模型和一氧化氮合酶（NOS）抑制型高血压模型中的影响,并初步探讨其作用机制。方法：将雄性清洁级小鼠随机分为对照组、模型组、别嘌呤醇组（A）和别嘌呤醇附子组（FA）。对照组小鼠灌胃给予蒸馏水,其余各组小鼠持续4周隔天灌胃给予腺嘌呤,从第3周开始灌胃给予相应药物即别嘌呤醇（70mg/kg）、附子水煎剂（腺嘌呤模型为5g/kg,L-NNA模型为3g/kg）。记录小鼠一般情况,第4周末测定小鼠血清一氧化氮（NO）,心组织蛋白、一氧化氮（NO）、丙二醛（MDA）,肝肾组织蛋白、一氧化氮（NO）、丙二醛（MDA）、尿酸（UA）含量。结果：与对照组相比,腺嘌呤模型组小鼠体重下降,摄食量减少,饮水量升高。与模型组相比,A组和FA组小鼠生化指标具有一定的改善,肾系数、小鼠血清、心、肝和肾组织NO和肝、肾组织UA、肝组织MDA降低了,肾组织MDA升高,其中FA组小鼠NO含量显著高于A组。L-NNA模型中,与对照组相比,模型组小鼠体重下降,摄食量和饮水量减少。与A组相比,FA组血清、心、肝和肾组织NO,肝和肾组织UA升高。在两种模型中,肝和肾组织中NO上升的幅度显著大于UA的上升幅度。综合结果表明腺嘌呤模型中FA组小鼠表现出显著的肾功能改善作用,L-NNA模型中FA组小鼠表现出肾功能损伤加重作用。结论：附子可能是通过激活肾素血管紧张素系统升高两种模型中血清、肝肾组织的NO含量。

The Effects and Mechanisms of Allopurinol Using Fuzi in The Two Kinds of Model

Objective： To observe the effects of allopurinol when Fuzi used in chronic renal failure model caused by adenine and hypertension model caused by nitric oxide synthase （NOS） inhibitory, and explore the related mechanisms preliminarily. Methods： Male ICR mice were randomly divided into 4 groups including control group, model group, allopurinol group （A）, Fuzi plus allopurinol group （FA）. The mice in control group and the other groups were given distilled water and adenine by intragastric adminis tration, respectively. The mice in A group and FA group were given the corresponding drugs once daily. The general condition of the mice were observed and the contents of nitric oxide（NO） in blood serum were determined. The hearts,livers and kidneys were homogenated for measuring nitric oxide （NO）, malonaldehyde（MDA）, uric acid （UA）. Results：Compared with control group, the body weight and food intake of mice in adenine model group decreased significantly, with drinking amount increased. Compared with model group, the biochemical indicators of mice in A group and FA group were improved , with kidney index, NO in blood serum, hearts,livers and kidneys, UA in livers and kidneys, MDA in livers reduced; while MDA in kidneys were raised. The NO in FA sig nifieantly higher than that in A. In hypertension model, compared with control group, the body weight, food intake and drinking amount of mice in model group decreased significantly. Compared with allopurinol group, the biochemical indicators of mice in FA group were improved , with NO in blood serum, hearts,livers and kidneys,UA in livers and kidneys raised. In the two models, the NO in liver and kidney tissue has been significant higher than the rise of UA. Comprehensive results show that the FA in adenine model showed a more significant role in improvement of renal function. While the FA in hypertension model showed aggravating renal damage effect. Conclusion： Fuzi may rise the NO in blood serum, liver and kidney through the activation renin angiotensin system.