硫化氢预处理晚期对大鼠缺血再灌注心肌Bcl-2、Bax蛋白表达及CK-MB水平的影响

目的研究硫化氢预处理晚期对大鼠缺血再灌注心肌的保护作用及其机制。方法将30只健康成年雄性S-D大鼠随机分为假手术组（Sham组）、缺血再灌注组（IR组）和硫化氢预处理组（HS组）,每组10只。Sham组仅分离左冠前降支,不阻断血流150 minI,R组阻断左冠前降支30 min,再灌注120 min,HS组静脉注射硫化氢供体NaHS 50μg/kg,24 h后同IR组处理。于实验结束时抽取动脉血检测肌酸激酶同工酶（CK-MB）水平;取左室免疫印迹法测Bcl-2、Bax蛋白表达水平。结果与IR组比,HS组CK-MB水平下降,Bcl-2蛋白表达增多,Bax蛋白表达减少（均P〈0.05）。结论硫化氢预处理晚期通过上调Bcl-2蛋白表达、下调Bax蛋白表达和减少CK-MB释放来减轻缺血再灌注损伤发挥心肌保护作用。

Effects of hydrogen sulfide delayed preconditioning on ischemia/reperfusion-induced myocardial Bcl-2/Bax protein expression and CK-MB level in rats

Objective To investigate the protective effect of hydrogen sulfide delayed preconditioning on myocardial ischemia reperfusion injury and the potential mechanisms in rats.Methods Thirty S-D adult male rats were randomly divided into sham group,IR group and HS group（N=10）.In sham group,we isolated left anterior descending（LAD） coronary artery,but not blocked for 150 minutes;in IR group,we blocked LAD for 30 min and then primed for 2h;in HS group,we injected donor of hydrogen sulfide—sodium hydrosulfide 50 μg/kg via vein,after 24h,dealed same with IR group.Arterial blood samples were taken to determine the concentration of creatine kinase MB at the end of experiment;the heart was harvested and level of Bcl-2/Bax protein expression was determined by Western Blotting.Results Compared with IR group,Bcl-2 protein expression was increased,Bax protein and CK-MB level were decreased in HS group（all P0.05）.Conclusion Hydrogen sulfide delayed preconditioning can protect ischemia/reperfusion-induced myocardium by regulating Bcl-2/Bax protein expression and CK-MB level in rats.