| Abstract: | To assess the role of bacterial adherence to uroepithelial cells in the pathogenesis of nosocomial urinary tract infection, we prospectively studied 55 patients with indwelling urinary catheters. We obtained uroepithelial cells from the bladder and urine for culture on the patients' entry into the study and every two to four days during catheterization. In all, 235 collections of uroepithelial cells from these patients were used in an in vitro adherence assay with six gram-negative bacterial strains. With uroepithelial cells from patients who did not have bacterial infections, the adherence of the bacteria used in the assay differed significantly according to species. The least adherence occurred with Escherichia coli GR12; the adherence increased with (in order) Proteus mirabilis, E. coli J96, Klebsiella pneumoniae, Serratia marcescens, and Pseudomonas aeruginosa. With cells collected just before the onset of bacteriuria, adherence of these gram-negative strains was higher in patients in whom gram-negative rod infections developed than in those with gram-positive coccal infections (P = 0.005). Analysis with the Cox proportional-hazards model demonstrated that a significant increase in bacterial adherence to uroepithelial cells in the bladder occurred two to four days before the onset of bacteriuria, but that adherence returned to base-line values with the onset of bacteriuria. These results suggest that a transient increase in the adherence of gram-negative bacteria to bladder epithelial cells may be an important early event in the development of catheter-associated bacteriuria. |
