三裂叶野葛茎段和叶柄培养成再生植株

三裂叶野葛的叶柄和茎段外植体在含有６－ＢＡ１．０ｍｇ／Ｌ和ＮＡＡ０．１ｍｇ／Ｌ的ＭＳ培养基上产生愈伤组织，愈伤诱导率分别为１００％和９４％，为进一步分化出芽，芽再生率分别为１００％和３３．３％，而在添加６－ＢＡ３．０ｍｇ／Ｌ和ＮＡＡ１．０ｍｇ／Ｌ的ＭＳ培养基中叶柄和茎段外植体的愈伤诱导率分别为１００％和６４．３％，芽再生率分别为５７．１％和２１．４％，茎长成嫩枝后转移至增加０．１ｍｇ／Ｌ ＮＡＡ的

Plantlet Regeneration from the Petiole and Stem of Pueraria phaseoloides

The callus induction rates on MS medium containing 6-BA 1.0 mg/L NAA 0.1 mg/L were 100% for petiole explant and 94% for stem explant. Callus could differentiate buds on the same medium, shoot regeneration rates were 100% and 33.3% respectively. While cultured in the medium supplemented with 6-BA 3.0 mg/L and NAA 1.0 mg/L, the callus induction rates were 100% and 64 3% respectively, and the shoot regeneration rates were 57.1% for petiole explant and 21.4% for stems. When the shoots were transferred onto MS medium containing 0.1 mg/L NAA, they differentiated into roots and formed regenerated plants. The survival rate was 90%.