多罗清泰颗粒对人肝癌细胞株信号转导基因表达的影响

目的观察多罗清泰颗粒对人肝癌细胞株SMMC-7721信号转导基因表达的影响。方法体内抑瘤实验研究多罗清泰颗粒对小鼠肝癌移植瘤生长的影响;采用中药血清药理学方法和基因芯片技术,分析多罗清泰含药血清对人肝癌细胞株SMMC-7721信号转导基因表达的影响。结果多罗清泰颗粒能显著抑制小鼠肝癌移植瘤生长,抑制率为63%~67.8%。多罗清泰颗粒对所检测的99条信号转导基因中的68条基因表达具有显著影响,占所测基因的69%。其中,上调的基因为42条,下调的基因为36条。在多罗清泰颗粒小剂量处理组和大剂量处理组之间,SMMC-7721细胞信号转导基因表达则存在相当大的差异。结论多罗清泰颗粒能显著抑制肝癌细胞的生长,显著影响肝癌细胞信号转导基因的表达。

Effect of Duoluoqingtai Granula on expression of sinal transduction gene in human hepatocellular carcinoma cell line

Objective To investigate the effect of Duoluoqingtai Granula (DLQTG) on gene expression in human hepatocellular carcinoma cell line (SMMC-7721). Methods Effect of DLQTG on the growth of mice-transplanted hepatocellular carcinoma was studied in vivo and on the expression of signal transduction genes of SMMC-7721 cell line with serum pharmacology and microarray. Results DLQTG was observed to significantly affect the growth of mice-transplanted hepatocellular carcinoma with inhibition rate from 63% to 67.8%, and the expression of signal transduction genes in 68 of 99 genes detected with up-regulation of 42 genes and down-regulation of 36 genes. There was obvious difference in the expression of signal transduction genes in SMMC-7721 cell line between low and high dosage of DLQTG-treated group. Conclusion DLQTG can be used to inhibit the growth of hepatocellular carcinoma and to affect the expression of signal transduction genes in human hepatocellular carcinoma significantly.