成年大鼠嗅球成鞘细胞的原代培养及形态学研究

目的探讨大鼠嗅球成鞘细胞(olfactoryensheathingcells,OECs)分离与纯化培养方法并对形态学进行研究。方法取材后采用差速贴壁和阿糖胞苷(Arac)抑制相结合的纯化培养方法培养嗅鞘细胞,并分不同阶段进行观察和NGFRp75和GFAP免疫组化染色鉴定。结果成功培养出高纯度的嗅鞘细胞,胞体呈梭形、多突起形及油煎蛋形三种类型,突起细长编织成网状为其特点。结论差速贴壁和Ara-c抑制相结合的纯化培养方法兼收了两者的优点,实践证实是一种简单经济而又有实效的嗅鞘细胞培养方法。

Primary culture and morphologic study of olfactory ensheathing cells from the adult rat olfactory bulb

Objective:To investigate an effective method of purification for establishing primary culture of olfactory ensheathing cells (OECs) from the adult rat olfactory bulb and the morphological changes of the cultured OECs. Method:OECs were harvested from olfactory bulbs and cultured,purified by the method of combining both the different rates of attachment among the various cells types and the Ara-c inhibition.The morphological changes of cultured OECs were observed at different stages and identified by both nerve growth factor receptor p75 (NGFRp75) and GFAP immunohistochemistry. Result: The cultured OECs presented three morphological types:fusiform,process-bearing multipolar,fried egg-like were high purity and their processes wove into net.Conclusion:The method of purification for OECs through combining both the different rates of attachment among the various cells types and the Ara-c inhibition is simple, inexpensive and practical.