| microRNA-449抑制脉络膜黑色素瘤细胞的增殖和迁移 |
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| 引用本文： | 王教,陈晓燕,瞿佳. microRNA-449抑制脉络膜黑色素瘤细胞的增殖和迁移[J]. 实用肿瘤杂志, 2009, 24(6): 535-538 |
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| 作者姓名： | 王教 陈晓燕 瞿佳 |
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| 作者单位： | 温州医学院眼视光学院,浙江,温州,325027 |
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| 摘 要： | 目的探讨脉络膜黑色素瘤细胞M21转染microRNA-449后对增殖和迁移的影响,及其分子机制的初探。方法采用阳离子脂质体Lipofectamine介导的方法,将microRNA-449转染入体外培养的人脉络膜黑色素瘤细胞。应用MTS法检测转染后细胞增殖的影响;Transwell小室检测细胞迁移的变化;Western blotting检测c-Met蛋白的表达。结果转染microRNA-449后,脉络膜黑色素瘤细胞增殖明显被抑制（P〈0.01）,迁移亦受抑制（P〈0.01）,c-Met表达量下降。结论转染microRNA-449对体外培养的人脉络膜黑色素瘤细胞的增殖和迁移具有抑制作用,microRNA-449可下调c-Met蛋白的表达水平。
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| 关 键 词： | 黑色素瘤/治疗 脉络膜肿瘤/治疗 微RNAs 基因疗法 增生 转染 基因表达 |
microRNA-449 inhibits proliferation and migration of uveal melanoma cells |
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| WANG Jiao,CHEN Xiao-yan,QU Jia. microRNA-449 inhibits proliferation and migration of uveal melanoma cells[J]. Journal of Practical Oncology, 2009, 24(6): 535-538 |
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| Authors: | WANG Jiao CHEN Xiao-yan QU Jia |
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| Affiliation: | (School of Ophthalmology and Optometry,Wenzhou Medical College,Wenzhou ,325027 ,China) |
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| Abstract: | Objective To investigate the effect and mechanism of microRNA-449 (miR-449) on proliferation and migration of uveal melanoma cells. Methods MicroRNA-449 was transfected into uveal melanoma cells. The proliferation and migration of uveal melanoma cells were examined by MTS and transwell migration assays,respectively. In addition,expression level of c-Met was determined by Western blotting. Results Transfection of miR-449 into uveal melanoma cells led to a significant decrease in cell growth and migration,and the expression of c-Met protein was downregulated. Conclusion miR-449 inhibits proliferation and migration of uveal melanoma cells through downregulation of c-Met. |
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| Keywords: | melanoma/ther choroid neoplasms/ther microRNAs gene therapy hyperplasia transfection gene expression |
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