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Antimicrobial activities of the methanol extract and compounds from the twigs of Dorstenia mannii (Moraceae) |
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| Authors: | Armelle T Mbaveng Victor Kuete Bathelemy Ngameni Veronique P Beng Bonaventure T Ngadjui Jacobus J Marion Meyer Namrita Lall |
| Affiliation: | 1. Clinical Trial Center for Functional Foods of Chonbuk National University Hospital, Geumam-dong, Deokjin-gu, Jeonju, 561-712, Republic of Korea 2. Department of Medical Nutrition Therapy, Chonbuk National University Medical School, Geumam-dong, Deokjin-gu, Jeonju, 561-180, Republic of Korea 4. Korea Food Research Institute, Baekhyeon-dong, Bundang-gu, Seongnam-City, 463-746, Gyeonggi-do, Republic of Korea 5. Department of Preventive Medicine, Chonbuk National University Medical School, Geumam-dong, Deokjin-gu, Jeonju, 561-180, Republic of Korea 6. Institute of Jinan Red Ginseng, Banwol-ri, Jinan-gun, 657-801, Republic of Korea 3. Department of Internal Medicine, Chonbuk National University Medical School, Geumam-dong, Deokjin-gu, Jeonju, 561-180, Republic of Korea |
| Abstract: | BackgroundRibosome-inactivating proteins (RIP) have been studied in the search for toxins that could be used as immunotoxins for cancer treatment. Pulchellin, a type 2 RIP, is suggested to induce immune responses that have a role in controlling cancer.MethodsThe percentage of dendritic cells and CD4+ and CD8+ T cells in the spleen (flow cytometry), cytokines?? release by PECs and splenocytes (ELISA) and nitric oxide production by PECs (Griess assay) were determined from tumor-bearing mice injected intratumorally with 0.1?ml of pulchellin at 0.75???g/kg of body weight. Statistical analysis was performed by one-way ANOVA with Tukey??s post hoc test.ResultsPulchellin-treated mice showed significant immune system activation, characterized by increased release of IFN-?? and Th2 cytokines (IL-4 and IL-10), while IL-6 and TGF-?? levels were decreased. There was also an increase in macrophage??s activation, as denoted by the higher percentage of macrophages expressing adhesion and costimulatory molecules (CD54 and CD80, respectively).ConclusionsOur results suggest that pulchellin is promising as an adjuvant in breast cancer treatment. |
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