卵巢癌基因1和癌超甲基化基因1蛋白在人卵巢上皮性肿瘤中的表达及临床意义

目的检测卵巢癌基因1(OVCA1)和癌超甲基化基因1(HIC1)蛋白在人卵巢上皮性肿瘤中的表达,并探讨其与卵巢上皮性肿瘤临床病理特征的联系。方法免疫印迹法检测20例正常卵巢组织、20例良性卵巢上皮性肿瘤组织、20例交界性卵巢上皮性肿瘤组织及39例卵巢上皮性癌组织中OVCA1和HIC1蛋白的表达。结果①卵巢癌和交界性卵巢肿瘤中OVCA1的蛋白表达量明显低于正常卵巢组织和良性卵巢肿瘤组织,差异有统计学意义(P<0.05)。②在卵巢癌中,低分化组中OVCA1的蛋白表达量低于高分化组,差异有统计学意义(P<0.05);临床Ⅲ+Ⅳ期中OVCA1的蛋白表达量低于临床Ⅰ+Ⅱ期,差异有统计学意义(P<0.05)。③与正常卵巢组织、良性卵巢肿瘤组织和交界性卵巢肿瘤组织相比,卵巢癌组织中HIC1的蛋白表达量显著降低,差异有统计学意义(P<0.05)。④HIC1蛋白表达量的变化与卵巢癌的病理类型、分级和临床分期无关。结论 OVCA1和HIC1蛋白表达水平的下调与卵巢上皮性癌的发生相关,OVCA1可能参与了卵巢上皮性癌的发展过程。

Expression and clinicopathological significance of OVCA1 and HIC1 in ovarian epithelial tumors

Objective To detect the expression of OVCA1 and HIC1 proteins in ovarian epithelial tumors and to explore the correlation between the expression and the clinicopathological features of ovarian epithelial tumors.Methods Western blot method was used to detect the expression of OVCA1 and HIC1 proteins in 20 cases of normal ovarian tissue,20 cases of benign ovarian epithelial tumor,20 cases of borderline ovarian epithelial tumor,and 39 cases of ovarian epithelial cancer.Results ①OVCA1 protein expression in ovarian cancer and borderline ovarian tumor was significantly lower than in normal ovarian tissue and benign ovarian tumor with statistical significance observed（P0.05）.②The expression of OVCA1 in poorly differentiated ovarian cancer was significantly lower than that in well differentiated tissues（P0.05）.The expression of OVCA1 in ovarian cancers of stages Ⅲ and Ⅳ was significantly lower than that in stages Ⅰ and Ⅱ cancers（P0.05）.③HIC1 protein expression was significantly lower in ovarian cancer than in normal ovarian tissue,benign ovarian tumor and borderline ovarian tumor（P0.05）.④The expression of HIC1 protein was not correlated with subtypes of ovarian cancer,pathological grading and clinical staging.Conclusion Down-regulated expression of OVCA1 and HIC1 proteins might play a role in the aetiology of ovarian cancer.Furthermore,OVCA1 might be involved in progression of ovarian cancer.