P27~(kip1) P16蛋白在人脑胶质瘤中的表达及相关性研究

目的探讨脑胶质瘤中P27kip1、P16蛋白表达改变与脑胶质瘤发生发展的相关性。方法应用免疫组织化学技术检测31例不同病理分级脑胶质瘤,5例正常脑组织为正常对照。结果 P16、P27kip1蛋白表达阳性率在低级别(Ⅰ、Ⅱ级)和高级别(Ⅲ、Ⅳ级)脑胶质瘤中分别为88%和35%,75%和22%。两种蛋白在低级别和高级别中的表达差异均具有统计学意义(P<0.05)。P16、P27kip1蛋白表达水平分别与脑胶质瘤病理分级之间呈负相关性(r=-0.483,P<0.01;r=-0.521,P<0.01),即随着肿瘤恶性度的升高而降低。P27kip1、P16蛋白在肿瘤中的表达呈相关(P=0.043)。结论 P27kip1、P16蛋白表达对脑胶质瘤的发生发展起重要作用,并且二者可能存在相互作用,它们异常表达将引起脑胶质瘤进一步向恶性演进。

Expression and correlation analysis of P27kip1 and P16 proteins in human glioma

Objective To investigate the altered expressions of P27^kip1 and P16 proteins as related to the development of brain stem glioma. Methods Immunohistochemistry was used to detect 31 specimens of brain stem glioma with various pathological grading and 5 of normal brain tissues. Results The positive expression rates of P16 and P27^kip1 protein in low （Ⅰ and Ⅱ） and high （Ⅲ and Ⅳ） pathological grades were 88% vs 35%, and 75% vs 22%, respectively, with significant differences in expressions of both proteins between grades （P0.05）. Expressions of P16 and P27^kip1 protein were negatively correlated with pathological grading （r=-0.483, P0.01; r=-0.521, P0.01）, with less expressions in highly malignant tumors. Besides, there was a significant correlation between expression of P27^kip1 and P16 proteins in glioma （P=0.043）. Conclusion This study suggested that the expressions of P16 and P27^kip1 protein may have important roles in the occurrence and development of human brain glioma. The altered expressions of P16 and P27^kip1 proteins may interact with each other and add to malignancy of brain glioma.