苦参碱抑制人子宫内膜癌细胞增殖转移及机制研究

目的探讨苦参碱对人子宫内膜癌细胞增殖转移的抑制作用及机制。方法 MTT法检测苦参碱对人子宫内膜癌Ishikawa细胞株的抑制作用;流式细胞术观察对细胞周期的影响;ELISA法检测细胞培养上清液中炎性因子IL-8的浓度;Western印迹法检测经苦参碱干预该细胞48h后,NF-кB信号途径中p65的变化。结果苦参碱可显著抑制Ishikawa细胞生长,并呈时间和剂量依赖性。苦参碱使细胞阻滞于G0/G1期。细胞培养上清液中IL-8的浓度随着干预时间延长而减少(P<0.05)。苦参碱作用48h后,细胞NF-кBp65表达明显下降(P<0.05)。结论苦参碱具有抑制人子宫内膜癌Ishikawa细胞增殖的作用,使细胞阻滞于G0/G1期;抑制子宫内膜癌转移的作用,可能与抑制IL-8分泌及阻断NF-кB信号通路有关。

Effects of Matrine on Proliferation and Metastasis of Human Endometrial Carcinoma Cell Line Ishikawa

Objective To study the effect of Matrine on inflammatory factor IL-8 and NF-кBp65 expression from human endometrial carcinoma cell line Ishikawa.Methods The changes of cell proliferation of Ishikawa cells treated with Matrine were observed by using MTT assay.The cell cycle expressions were determined by flow cytometry.The densities of IL-8 in the supenmatant fluid were measured by ELISA.And NF-кBp65 were determined by western blot assay.Results Matrine could inhibit the proliferation of Ishikawa cells in vitro significantly in a dose and time dependent manner（P0.05）.The G0/G1 phase arrest was induced by treatment after 48 hours（P0.05）.The densities of IL-8 in the supenmatant fluid were decreased.Results revealed that cellular NF-кBp65 levels decreased in Matrine-treated cells at 48 hours（P0.05）.Conclusion Matrine could inhibit Ishikawa cell proliferation,induce G0/G1 phase arrest and by interfering with the NF-кB signaling pathway.