MCP-1启动子-2518位点基因多态性与云南汉族SLE的研究

目的研究单核细胞趋化蛋白-1（MCP-1）启动子-2518位点基因多态性与云南汉族系统性红斑狼疮（SLE）及狼疮性肾炎（LN）的联系。方法采用聚合酶链反应（PCR）与限制性片段长度多态性（RFLP）方法对61例SLE患者（SLE合并LN患者28例，SLE未合并LN患者33例）和65例正常对照的MCP-1启动子-2518位点基因多态性分布进行分型，以SPSS12．0分析软件分析该基因位点多态性情况。结果各组MCP-1启动子-2518位点基因型A／G较A／A、G／G出现的频率升高；各组MCP-1启动子A／A、G／G及A／G出现的频率无显著性差异（P〉0．05）。结论MCP-1启动子-2518位点基因多态性与SLE、LN的发病和病情之间均无联系。

Study on the association between MCP-1-2518 promoter polymorphism and systemic lupus erythematosus in Chinese Han in Yunnan Province

Objective To investigate the association between systemic lupus erythematosus(SLE) and the polymorphism of MCP-1-2518 promoter in chinese han patients from Yunnan Province.Methods Genotypes of MCP-1 promoter were determined by polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP) in 62 patients with SLE(29 were SLE with lupus nephritis(LN),33 were SLE with no LN) and 65 normal controls,the difference between each group had been compared.Results The AG genotype was more common in each group.The frequency of A/G was no significant difference among three groups(P>0.05).Conclusion These results suggest that there were no association between MCP-1 and the polymorphism in the MCP-1-2518 promoter and SLE or LN.