大肠癌中CD44V6、p53、PCNA的表达及意义

目的　探讨 CD44 V6、p5 3、PCNA在大肠癌中的表达与病理及生理学行为之间的关系。方法　采用免疫组化S- P法检测 CD44 V6、p5 3、PCNA在 5 1例大肠癌中的表达。结果　 5 1例大肠癌 CD44 V6阳性表达率 41.18% (2 1/ 5 1)。CD44 V6在低分化大肠癌的检出率为 5 3.85 % (7/ 13)明显高于高分化大肠癌 36 .84% (7/ 19) (P<0 .0 5 ) ,在浆膜外的阳性表达率 5 3.38% (8/ 15 )明显高于肌层 33.33% (2 / 6 ) (P<0 .0 5 ) ,有淋巴结转移者阳性表达率为 6 3.16 % (12 / 19)明显高于无淋巴结转移者 31.2 5 % (10 / 32 ) (P<0 .0 5 )。p5 3在 5 1例大肠癌中阳性检出率为 5 0 .5 9% (2 6 / 5 1) ,其表达与大肠癌的组织类型、分化程度和淋巴结转移未见明显相关。而与浸润深度呈正相关 ,p5 3在浆膜外层的阳性表达率为5 3.33% (8/ 15 )明显高于肌层 33.33% (2 / 6 ) (P<0 .0 5 )。 PC-NA的表达与病理分级、浸润深度和淋巴结转移未见显著相关性 (P>0 .0 5 )。结论　 CD44 V6的表达与大肠癌的临床病理生理学行为密切相关 ,而 p5 3与大肠癌浸润深度关系密切。因此 CD44 V6和 p5 3蛋白可为一个准确预测大肠癌预后的生物学指标。而 PCNA作为大肠癌预后的标记物不够理想

RELATIONSHIP BETWEEN EXPRESSION OF CD44V6,P53,PCNA AND THE PROGNOSIS OF COLORECTAL CARCINOMA

Objective To study the relation between the expression of CD44V6, p53 and PCNA and the prognosis of colorectal carcinoma. Methods Immnohistochemical method was used to detect the expression or CD44V6, p53 and PCNA in 51 patients with colorectal cacinoma.Results The positive rate of CD44V6 was 41.18%(21/51). It was 53.85%(7/13) in low differentiated colorectal carcinoma,markedly higher than that in highly differemtiated carcinoma (36.84%,7/19)( P <0 05) The expression rate outside of serosa was 53.34%(8/15), markedly higher than that in muscular layer (33.33%,2/6)( P <0 05)Also, it was much higher in carcinoma with lymph node metastasis (63.16%, 12/19) than that in carcinoma without lymph node metastasis (31.25 %,10/32)( P <0 05).The positive rate of p53 was (50.59% 26/51) and was only related with the depth of infiltration of carcinoma. The expression of p53 in the outer layer of serosa was (53.33%, 8/15),apparently higher than that in muscular layer (33.33%,2/6)( P <0 05),while the expression of PCNA was not related to pathological grade, depth of infiltration and lymph node metastasis ( P <0 05).Conclusion The expression of CD44V6 and p53 is correlated with progression and metastasis of colorectal carcinoma, so CD44V6 and p53 may be used as a hopeful marker for predicting the metastastic potential of colorectal carcinoma and the prognosis of these patients.