IL-10在大鼠心肌缺血再灌注中的变化及意义

目的探讨大鼠心肌缺血再灌注过程中血清IL-10浓度及意义,为临床诊断IRI提供实验依据。方法将大鼠随机分成假手术组(对照组,n=21),缺血/再灌注(模型组)和甲基强的松龙治疗组(药物组,n=21)3组,每组内再随机分成缺血0.5h(n=7)、再灌注0.5h(n=7)再灌注2h(n=7)三个亚组。在心肌缺血前用甲基强的松龙(30mg/kg)对药物组大鼠预处理。分别测定缺血0.5h、再灌注0.5h及2h血清中IL-10的含量和心肌组织中MPO含量。结果①对照组IL-10无明显变化,模型组与药物组自缺血0.5h、再灌注0.5h至2hIL-10呈逐渐升高趋势,差异具有显著性意义(模型组:F=26.075,P<0.01;药物组:F=39.263,P<0.01),两两比较差异具有显著性意义(P<0.05);各时段内,自对照组、模型组、药物组IL-10明显升高,差异具有显著性意义(缺血0.5h:F=85.383,P<0.05;再灌注0.5h:F=64.923,P<0.01;再灌注2h:F=131.727,P<0.01),两两比较差异具有显著性意义(P<0.05)②对照组心肌组织MPO活性无明显变化;模型组和药物组中,心肌组织MPO含量随着再灌注时间延长而升高,并随着再灌注时间进一步延长而略有降低,差异具有显著性意义;模型组F=4.153,P<0.05;药物组F=8.725,P<0.01);相应时段内,对照组、药物组、模型组差异具有显著性意义(缺血0.5h:F=403.785,P<0.01;再灌注0.5h:F=119.8

Change and significance of endogenous Interleukin-10 during myocardial ischemia and reperfusion in rats

Objective To investigate the changes and relations of serum IL-10 levels of myocardium myeloperoxidase (MPO) during acute myocardial ischemia and reperfusion in rats in order to supply experiment basis for clinical diagnosis. Methods 63 rats were divided into three groups at random, including 21 control rats, 21 model rats who received i.v. bolus placebo and 21 administration rats who received i.v. bolus methylprednisolone before ischemia. Among them model and administration groups subjected to myocardial ischemia and reperfusion. Plasma IL-10 by ELISA was measured at 0.5 hour after ischemia and 0.5, 2 hours after reperfusion, meantime myocardial myeloperoxidase (MPO) were measured at respective time points. Results IL-10 change little in control group. Significant levels of IL-10 were showed in both model and administration groups from ischemia 0.5 hour to 0.5 hour, 2 hours after myocardial reperfusion(model group:F=26.075,P<0.01; administration group: F=39.263,P<0.01). Levels of IL-10 increased significantly from control group to model group, administration group at same time point(ischemia 0.5 h: F= 85.383, P<0.05; I/R 0.5 h: F=64.923, P<0.01; I/R 2h: F=131.727, P<0.01) .MPO significantly rose following prolonging reperfusion time (MPO: model group:F=4.153, P<0.05; administration group: F=8.725, P<0.01), but declined slightly with more reperfusion extending. Concentrations of MPO in administration group were lower than those in model group at respective time point(P<0.05). The expression of IL-10 neglect correlated with MPO after reperfusion(P<0.05). Conclusions The present findings indicated that mythylprednisolone enhanced highly release of endogenous IL-10. Level of IL-10 showed indirectly scale of inflammation during the ischemia and reperfued myocardia.