BAG-1、CDK4蛋白在结直肠癌中的表达及意义

目的：探讨BAG-1、CDK4蛋白在结直肠癌中的表达及其与结直肠癌临床病理特征的关系。方法：应用免疫组化SP法对80例结直肠癌组织、40例结直肠腺瘤组织及25例癌旁正常结直肠组织中的BAG-1、CDK4进行检测。结果：BAG-1、CDK4在结直肠癌中过度表达，阳性率分别为80．0％（64／80）和65．0％（52／80），并且BAG-1、CDK4与肿瘤的分化程度成反比。有淋巴结转移的结直肠癌BAG-1、CDK4的阳性率分别为96．7％（29／30）和83．3％（25／30）．无淋巴结转移的结直肠癌阳性率分别为70．0％（35／50）和54．0％（27／50），两者比较，差异有高度统计学意义（P〈0．01）。侵及浆膜层者CDK4蛋白表达阳性率明显高于未侵及浆膜层者，阳性率分别为71．4％（40／56）和35-3％（12／34），差异有高度统计学意义（P〈0．01）。结论：BAG—1、CDK4的过度表达与肿瘤的分化程度、淋巴结转移密切相关；CDK4的过度表达还与浸润深度密切相关；结直肠癌的发生机制涉及BAG-1、CDK4调节环路中多个基因的异常。

Expression and significance of BAG-1 and CDK4 in the colorectal cancer

Objective： To explore the expressions of BAG-1 and CDK4 protein in eolorectal cancer and its relationship with clinicopathological features of eoloreetal cancer. Methods： BAG-1 and CDK4 protein were detected in 80 cases of eoloreetal cancer, 40 cases of eolorectal adenomas and 25 cases of adjacent normal eolorectal tissues by immunohistoehem istry SP method. Results： The overexpression of BAG-1 and CDK4 in coloreetal cancer were 80.0% （64/80） and 65.0% （52/80） respectively, BAG-1 and CDK4 were inversely proportional to the degree of tumor differentiation. The positive rates of BAG-1 and CDK4 were 96.7% （29/30） and 83.3% （25130） in lymph node metastasis of colorectal cancer, and were 70.0% （35/50） and 54.0% （27/50） in no lymph node metastasis of coloreetal cancer, they showed significant difference （P〈0.01）. CDK4 protein expression in serosal invasion was significantly higher than those less than serosal layer, the positive rates were 35.3% （12/34） and 71.4% （40/56） respectively, they were statistically significant （P〈0.01）. Conclusion： The overexpression of BAG-1 and CDK4 shows a good correlation with tumor differentiation and lymph node metastasis. The overexpression of CDK4 is closely related with the depth of invasion. Abnormality of many genes in the regulatory pathway of BAG-1 and CDK4 may be involved in the molecular mechanism of colorectal carcinogenesis.