Egr-1基因在人宫颈癌Hela细胞中的表达及其对细胞凋亡的作用

目的　研究不同剂量60 Co射线照射后Egr 1基因在人宫颈癌Hela细胞中的表达及与细胞凋亡的关系。方法　将人宫颈癌Hela细胞株进行体外培养 ,用不同剂量的60 Co射线照射 ,流式细胞仪记录细胞周期的分布和凋亡率 ,电子显微镜观察细胞形态 ,半定量RT PCR法检测Hela细胞Egr 1基因的表达。结果　放射线可抑制人宫颈癌Hela细胞的生长 ,诱导其凋亡。照射后细胞凋亡率随照射剂量增加而增加 ,至 12 0 0cGy时达高峰值 ,与对照组相比均有极显著性差异 (P <0 .0 1) ;照射后Hela细胞出现G0 /G1期阻滞 ,S期明显减少 ,且呈剂量依赖性 ,与对照组相比均有极显著性差异 (P <0 .0 1) ;照射后Egr 1基因表达随照射剂量增加而增加 ,至 12 0 0cGy时达峰值 ,与对照组比均有极显著性差异 (P <0 .0 1) ,Egr 1基因表达与凋亡率相关 (r=0 .96 9,P <0 .0 1)。结论　60 Co射线可引起人宫颈癌Hela细胞周期阻滞并诱导细胞凋亡 ,Egr 1基因为促凋亡基因 ,可能与宫颈癌的发生、发展及预后有关

Expression of Egr-1 gene in Hela cells and its effect on apoptosis

Objective To investigate the relationship between apoptosis and the expression of Egr-1 gene after different dose of radiation.Methods Hela cell lines cultured in vitro were exposed to 60Co with different dose of radiation.The treated cells were observed under flow cytometry for distribution of cell cycle and rate of apoptosis,transmission electron microscopy for cell morphology,the expression of Egr-1 gene was detected by semi-quantitative RT-PCR.Results Radiation inhibited Hela cells proliferation and induced its apoptosis.After irradiation the apoptosis ratios increased with different dose of radiation,when the dose achieved 1 200 cGy,the apoptosis ratio reached the peak,and were significantly higher than that in control (P<0.01).Hela cells showed significant dose-dependent G 0/G 1 phase arrest and S phase decrease after irradiation,and were significantly higher than those in control (P<0.01).The mRNA expression of Egr-1 gene increased with different radiation dose,and were significantly higher than that in control (P<0.01).The mRNA expression of Egr-1 gene was significantly correlated with apoptosis ratio(P<0.01).Conclusion 60Co rays can induce cell cycle arrest and apoptosis in Hela cells.The development of apoptosis in Hela cells has a promoting regulating function to Egr-1 gene.