动脉灌注酸性成纤维细胞生长因子治疗兔股骨头缺血性坏死模型

目的 探讨动脉灌注酸性成纤维细胞生长因子(aFGF)治疗兔股骨头缺血性坏死模型的价值。方法 以液氮冷冻法建立兔股骨头缺血性坏死模型30只,建模后4周分为3组行DSA引导下动脉灌注。实验组12只,动脉灌注aFGF;药物对照组12只,动脉灌注尿激酶、丹参、罂粟碱;假手术对照组6只,动脉灌注生理盐水。动脉灌注后4周,对3组行股骨头区造影及骨密度检测后,处死实验兔行组织学检查,记录空骨陷窝阳性数。另对实验组于动脉灌注前及灌注后4周处死前检测肿瘤标志物。结果 造影示实验组股骨头区血管及侧支循环明显多于药物对照组及假手术对照。骨密度检测示实验组骨密度(0.65±0.03)明显高于药物对照组(0.55±0.03)及假手术对照组(0.44±0.04,P均<0.05)。动脉灌注前及灌注后,实验组各肿瘤标志物检测均为阴性。组织学检查示实验组空骨陷窝阳性数(13.92±1.67)明显低于药物对照组(18.50±1.45)及假手术对照组(23.17±1.94,P均<0.05)。结论 动脉灌注aFGF可促进股骨头区侧支循环建立,加速液氮冷冻兔股骨头坏死模型的修复,较动脉灌注常规药物作用更为明显。

Arterial perfusion of acidic fibroblast growth factor in the treatment of femoral head ischemic and necrosis in rabbit models

Objective To analyze the value of perfusion acidic fibroblast growth factor (aFGF) through artery in the treatment of avascular necrosis of the femoral head (ANFH) in rabbit models. Methods The ANFH medols were established in 30 rabbits by liquid nitrogen frostbiting. After 4 weeks, the medols were divided into 3 groups. There were 12 in experimental group with aFGF perfused by arterial approach, 12 in medicine control group with perfusion of urokinase, salvia miltiorrhiza and papaverine, 6 in sham control group with perfusion of normal saline. Four weeks after perfusion, angiography of femoral head and measurement of bone mineral density (BMD) were performed. Then the rabbits were killed for histological examination. The number of empty lacunae by means of HE was counted. In experimental group, tumor markers were detected before perfusion and 4 weeks after perfusion. Results The collateral vessel of left femoral region in experimental group was significantly better than another two groups. Also BMD of experimental group (0.65±0.03) was higher than medicine control (0.55±0.03) and sham control groups (0.44±0.04, both P<0.05). Meanwhile, the tumor markers were undetectable before and after perfusion in experimental group. There were less number of empty lacunae in the experimental group (13.92±1.67) than medicine control (18.50±1.45) and sham control groups (23.17±1.94, both P<0.05). Conclusion Perfusion of aFGF through artery can promote the formation collateral vessel of femoral region and accelerate the repairing process of ANFH medols in rabbits. There is better effect of perfusing aFGF than general medicine.