宫颈癌及宫颈上皮内瘤变MGMT基因甲基化及其相关性研究

目的：探讨宫颈癌和宫颈上皮内瘤变（CIN）患者宫颈脱落细胞中O6-甲基鸟嘌呤- DNA甲基转移酶（MGMT）基因启动子区甲基化状态在宫颈癌临床早期诊断和筛查中的作用。方法选取对照组20例，CINI、CINII、CINIII期患者各50例和宫颈癌患者50例，采用甲基化特异性PCR检测宫颈脱落细胞中MGMT基因启动子区甲基化状态并进行比较。结果宫颈癌和CINI、II、III期患者MGMT基因启动子区甲基化阳性率分别为82.0%（41/50）、24.0%（12/50）、50.0%（25/50）和80.0%（40/50），而对照组未检测到MGMT基因启动子区甲基化。与对照组比，宫颈癌组和CINI、II、III期组MGMT基因启动子区甲基化阳性率的差异均有统计学意义（均P＜0.05）。宫颈癌组中MGMT基因启动子甲基化与其临床分期及组织分化程度有显著相关性（均P＜0.05）。结论 MGMT基因启动子区甲基化可能参与宫颈癌的发生、发展，有助于宫颈癌的早期辅助诊断和筛查。

Relationship between methylation of MGMT gene promoter and cervical carcinoma

Objective To investigate the methylation status of MGMT gene promoter in cervical carcinoma and cervical intraepithelial neoplasia （CIN）. Methods Methylation status of MGMT gene promoter was detected with methylation specific PCR （MSP） in cervical exfoliated cel s from patients with CINI （n=50）, CINII （n=50）, CINIII （n=50）, cervical carcinoma （n=50） and non- cancer controls（n=20）. Results The promoter methylation rates of MGMT were 82.0%（41/50）, 24.0%（12/50）, 50.0%（25/50） and 80.0%（40/50） in cervical carcinoma, CIN I, CINII and CINIII, while no methylation was detected in control group （P〈0.05）. The methylation of MGMT gene was correlated with the clinicopathological characteristics and tissue differentiation of cervical carcinoma （P〈0.05）. Conclusion The methylation of MGMT gene promoter region is associated with cervical carcinoma, which may be used as potential marker for early diagnosis of cervical carcinoma.