骨髓间充质干细胞经肾动脉移植修复小鼠输尿管损伤的方法学研究

目的：从方法学上研究骨髓间充质干细胞(bone marrow mesenchymal stem cells，BMSCs)经肾动脉移植对小
鼠输尿管损伤的修复作用，探讨经肾动脉移植BMSCs的有效性。方法：显微血管夹夹毕49只3~4周龄Balb/c小鼠的
左侧输尿管，10 d后取出显微血管夹以解除左侧输尿管梗阻，然后将这些实验小鼠随机分为实验组(n=25)及对照组
(n=24)。实验组小鼠取出显微血管夹后立即经肾动脉移植荧光素酶标记的Balb/c小鼠BMSCs，对照组小鼠取出显微血
管夹后随即关闭切口，不移植BMSCs。磁共振成像对实验小鼠进行扫描，通过测量不同时间点的实验小鼠的左肾盂
容积，比较实验组与对照组小鼠左侧输尿管梗阻解除后的左输尿管复通率，评估BMSCs对输尿管损伤的修复作用。
结果：实验小鼠左侧输尿管梗阻后的左肾盂容积明显大于梗阻前(P﹤0.01)；实验组与对照组小鼠左侧输尿管梗阻后10
d的左肾盂容积差异无统计意义(P=0.693)；实验组小鼠左侧输尿管梗阻解除后的左输尿管复通率较对照组小鼠明显增
高(P=0.012)。结论：BMSCs经肾动脉移植可促进小鼠输尿管损伤的修复。

Methodology of BMSCs in repairing ureteral injury in mice via renal artery transplantation

Objective: To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on ureteral
injury repair via renal artery transplantation.
Methods: The left ureteral obstruction model was set up in 49 Balb/c mice by micro vascular clamp. The
microscopic vascular clamp was taken out to lift the left ureteral obstruction after 10 days. The mice were
randomly divided into an experimental group (n=25) and a control group (n=24). Balb/c mice BMSCs transfected by luciferase (Luc) were transplantated immediately through the renal artery after removing
the microscopic vascular clamp from the experimental group; while mice in the control group was
closed the incision after the microscopic vascular clamp was removed immediately and without BMSCs
transplant. Magnetic resonance imaging (MRI) was used to scan the experimental mice. By measuring
the left renal pelvis volume of the experimental mice at different time points and comparing the left ureter
recanalization rate after removing left ureteral obstruction of the experimental group and the control group,
we evaluated the repair effect of BMSCs on ureteral injury.
Results: The volume of the left renal pelvis in experimental mice became bigger obviously after the
left ureter was obstructed (P﹤0.01). The left renal pelvis volume of the experimental group and the
control group had no statistical significance 10 days after the left ureteral obstruction was set up
(P=0.693). In the experimental group, the left ureter recanalization rate was higher than that in the
control group, after removing the left ureteral obstruction (P=0.012).
Conclusion: Transplantation through the renal artery can promote the restoration of ureteral
injury in mice.