试管地黄诱导技术的研究

目的 :研究小植株离体条件下试管地黄诱导的最佳培养基和培养条件。方法 :选用地黄“855”为试验材料 ,研究了植物激素、蔗糖浓度、活性炭和日照时间等对地黄试管苗不定根的形成及膨大的影响。结果与结论 :将试管苗先在 1/2MS +IBA 1mg·L^-1的培养基中诱导生根后 ,转入诱导培养基中 ,根可膨大形成试管地黄 ,其最适诱导培养基为MS+6BA2mg·L^-1+NAA 0.1mg·L^-1+蔗糖 5% ,最适培养条件为 25℃、光照 2000～3000lx ,12h·d^-1,培养基中不宜添加活性炭和GA3 。

Microtuber Induction in Vitro from Rehmannia glutinosa

OBJECTIVE: To set up the fittest medium and optimum condition for plantlets to form microtubers. METHOD: Plant hormones, concentration of sucrose, active carbon and light time influenced formation of adventitious root and microtuber in vitro from plantlet of Rehmannia glutinosa (85-5). RESULT AND CONCLUSION: The plantlets were cultivated on the medium of microtuber induction after roots were induced on the 1/2MS medium with IBA 1 mg.L-1. The best medium was MS + 6-BA 2 mg.L-1 + NAA 0.1 mg.L-1 + Sucrose 5%. The fittest incubation temperature was 25 degrees C and the light length was (2,000-3,000 lx) 12 h.d-1. Active carbon and GA3 should not be added to the medium, which was not suitable for the induction of microtuber.