Tiam1表达与大肠癌外周血微转移的关系

目的〓〖HTK〗探讨Tiam1与大肠癌外周血微转移的关系。〖HTW〗方法〓〖HTK〗采用实时荧光定量逆转录PCR方法检测51例大肠癌患者外周血微转移标志物CK20 mRNA的表达以及Tiam1在大肠癌组织及距肿瘤边缘5～10cm的正常粘膜组织中的表达，应用SPSS11.5统计软件分析Tiam1 mRNA表达水平与大肠癌临床表现及病理特征的相关性。〖HTW〗结果〓〖HTK〗51例中18例（35.29%）检测到外周血微转移。Tiam1 mRNA在有外周血微转移的大肠癌组织及无外周血微转移的大肠癌组织中表达分别为（72.11±2.38）×10-4、（15.78±5.01）×10-4， 差异有统计学意义（P＜0.05）。大肠癌Tiam1 mRNA的表量与患者的性别、年龄、原发部位、肿瘤直径、外周血CEA值无相关关系（P>0.05）, 但与肿瘤外周血微转移、分化程度、浸润深度、淋巴结转移、远处转移、TNM分期相关（P<0.05）。〖HTW〗结论〓〖HTK〗Tiam1与大肠癌外周血微转移、组织分化、淋巴结转移密切相关，检测Tiam1 mRNA含量有助于判断大肠癌的侵袭和转移，尤其是外周血微转移。

Relationship between Tiam1 expression and peripheral blood micro-metastasis in colorectal carcinoma

Objective〓〖WTBZ〗To explore the role of T lymphoma invasion and metastasis factor 1(Tiam1) expression in determining invasion and metastasis, especially the peripheral blood micro-metastasis in colorectal carcinoma. 〖WTHZ〗Methods〓〖WTBZ〗Real-time fluorescence quantitative-polymerase chain reaction (FQ-RT-PCR) was used to determine the expressions of CK20 mRNA and Tiam1mRNA in 51 cases of colorectal carcinoma, and software SPSS 11.5 was used to analyze the relationships between colorectal carcinoma clinical and pathological features and Tiam1 expression. 〖WTHZ〗Results〓〖WTBZ〗CK20 mRNA expression was found in 18 cases(35.29%, 18/51), and Tiam1 mRNA was found in all, however it was （72.11±2.38）×10-4 in cancerous tissues with micro-metastasis and was（15.78±5.01）×10-4 in cancerous tissues without micro-metastasis, and the difference was statistically significant (P＜0.05). Tiam1 expression had no relations to age, gender, primary location, tumor size and peripheral blood carcino-embryonic antigen level , but had relations to tumor differentiation and invasion, lymphocyte metastasis, tumor TNM stage and peripheral blood micro-metastasis （P<0.05）. 〖WTHZ〗Conclusion〓〖WTBZ〗Tiam1mRNA determination is helpful in judging invasion and metastasis of colorectal carcinoma, especially in clarifying peripheral blood micro-metastasis.