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| HPV-16 E6基因沉默对宫颈癌CaSki细胞的影响 | |
| 引用本文： | 成海恩,张溢,张翠莉,潘巍巍,石华,何柳兴,易发平,宋方洲.HPV-16 E6基因沉默对宫颈癌CaSki细胞的影响[J].第四军医大学学报,2006,27(20):1888-1891. |
| 作者姓名： | 成海恩 张溢 张翠莉 潘巍巍 石华 何柳兴 易发平 宋方洲 |
| 作者单位： | 1. 重庆医科大学,生物化学与分子生物学教研室,重庆,400016;重庆医科大学,生物化学与分子药理学重点实验室,重庆,400016;重庆医科大学,教育部临床检验诊世学重点实验室,重庆,400016 2. 重庆医科大学,生物化学与分子生物学教研室,重庆,400016 3. 重庆医科大学,公共卫生学院生殖医学教研室,重庆,400016 4. 重庆医科大学,生物化学与分子药理学重点实验室,重庆,400016 |
| 摘 要： | 目的: 研究特异性抑制HPV E6基因的表达对宫颈癌CaSki细胞的影响. 方法: 构建表达HPV-16 E6基因siRNA重组质粒,体外转染CaSki细胞后检测E6 mRNA表达变化及E6主要作用靶分子P53的蛋白变化;流式细胞术分析细胞周期. 结果: 特异RNAi表达质粒转染CaSki细胞后明显降低了E6 mRNA水平,且P53蛋白水平增高. 流式细胞仪测定发现细胞停滞在G0-G1期,细胞增殖受到抑制. 结论: 采用RNA干扰技术能沉默CaSki细胞中整合的HPV-16 E6基因的表达,使细胞中抑癌蛋白P53水平增高,导致肿瘤细胞生长停滞. |
| 关 键 词： | RNA干扰 宫颈肿瘤 乳头状瘤病毒 人 E6基因 |
| 文章编号： | 1000-2790(2006)20-1888-04 |
| 修稿时间： | 2006年4月10日 |
Effect of silencing human papillomavirus 16 E6 gene on CaSki cell line |
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| CHENG Hai-En,ZHANG Yi,ZHANG Cui-Li,PAN Wei-Wei,SHI Hua,HE Liu-Xing,YI Fa-Ping,SONG Fang-Zhou.Effect of silencing human papillomavirus 16 E6 gene on CaSki cell line[J].Journal of the Fourth Military Medical University,2006,27(20):1888-1891. | |
| Authors: | CHENG Hai-En ZHANG Yi ZHANG Cui-Li PAN Wei-Wei SHI Hua HE Liu-Xing YI Fa-Ping SONG Fang-Zhou |
| Abstract: | AIM: To study the effect on CaSki cells by inhibiting human papillomavirus (HPV)16 E6 gene expression in CaSki cell. METHODS: The vectors expressing siRNA of HPV-16 E6 gene were constructed and transfected into CaSki cells. The expression of E6 mRNA was detected by RT-PCR and the expression of P53 protein by Westen blot. CaSki cell cycle was analyzed by flow cytometry. RESULTS: After transfection, the expression of HPV-16 E6 mRNA was reduced and the expression of P53 was up-regulated in CaSki cell line. The proliferation of CaSki cells were inhibited and the cells were arrested at G0-G1 stage. CONCLUSION: RNAi can inhibite the expression of HPV-16 E6 gene in CaSki cells, which up-regulates the expression of tumor suppression protein P53 and results in growth retardation of CaSki cells. |
| Keywords: | RNA interference cervix neoplasms papillomavirus human E6 gene |
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