丙型肝炎分子生物学、血清学诊断方法的研究和临床应用

STUDY AND CLINICAL APPLICATION OF MOLECULAR BIOLOGICAL AND SEROLOGICAL DIAGNOSTIC METHOD OF HEPATITIS C VIRUS

Using techniques of molecular biology and serology, we research on the establishment of HCV diagnotic quality control standard, HCV diagnostic materials, anti-HCV EIA diagnostic method, detection of serum HCV RNA,detection of HCV RNA in liver tissue by in situ PCR, and the establishment of monoclonal hybridoma cell line of HCV C33c and NS5a. Successfully prepared diagnostic kits have applied in screening of blood donor, clinical diagnosis,observation of treatment effect and research on pathogenesis of hepatitis C. After the use of these reagents, the transmission rate of HCV through blood product has been dramatically reduced.