| 分子杂交检测人血清中乙肝病毒DNA |
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| 引用本文： | 李洪,王清纯,潘兴玉,罗文炽,吴民泸,陈文缘. 分子杂交检测人血清中乙肝病毒DNA[J]. 泸州医学院学报, 1990, 0(1) |
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| 作者姓名： | 李洪 王清纯 潘兴玉 罗文炽 吴民泸 陈文缘 |
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| 作者单位： | 泸州医学院生物化学教研室，泸州医学院生物化学教研室，泸州医学院生物化学教研室，泸州医学院生物化学教研室，泸州医学院生物化学教研室，泸州医学院生物化学教研室 |
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| 摘 要： | 作者采用放射性同位素~(32)P标记的HBV-DNA探针,与固定在硝酸纤维膜上的乙肝病杂交,然后经过放射自显影测人血清中的乙病肝毒,取得了满意的结果,可在临床检验中推广应用。
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| 关 键 词： | 乙型肝炎病毒 脱氧核糖核酸 病毒 斑点试验 |
DETECTION OF HEPATITIS B VIRUS DNA IN HUMAN SERUM BY A MOLECULAR HYBRIDIZATION TECHNIQUE |
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| Li Hong. DETECTION OF HEPATITIS B VIRUS DNA IN HUMAN SERUM BY A MOLECULAR HYBRIDIZATION TECHNIQUE[J]. Journal of Luzhou Medical College, 1990, 0(1) |
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| Authors: | Li Hong |
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| Affiliation: | Li Hong Department of Biochemistry |
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| Abstract: | The authors report a method, by which hepatitis B virus DNA(HBV-DNA)in human serum could be detected sensitively and specificolly. This method involves an HBV-DNA probe labeled by~(32)P which is used to hybridize with the samples containing HBV-DNA which had been fixed on nitrocellulose fiiters. By means of a radioautography technique, the HBV-DNA positive sera could be determined. This method might be applied to clinical tests very conveniently. |
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| Keywords: | Hepatitis Bvirus DNA virus |
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