| 454 GS Junior测序平台用于细菌基因组de novo测序的评价 |
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| 引用本文： | 陈晨,张媛媛,王海印. 454 GS Junior测序平台用于细菌基因组de novo测序的评价[J]. 中国医药指南, 2013, 0(14): 1-2 |
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| 作者姓名： | 陈晨 张媛媛 王海印 |
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| 作者单位： | 陈晨 (中国疾病预防控制中心传染病预防控制所/传染病预防控制国家重点实验室,北京,102206)； 张媛媛 (中国疾病预防控制中心传染病预防控制所/传染病预防控制国家重点实验室,北京,102206)； 王海印 (中国疾病预防控制中心传染病预防控制所/传染病预防控制国家重点实验室,北京,102206)； |
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| 基金项目： | 国家自然科学基金项目(81201322)；传染病预防控制国家重点实验室面上课题(项目编号：2011SKLID206) |
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| 摘 要： | 目的评价454 GS Junior测序平台在细菌基因组测序中的应用价值。方法利用454 GS Junior测序平台测定1株霍乱弧菌的基因组序列,组装注释后,与参考序列霍乱弧菌O1埃尔托生物型菌株N16961基因组序列进行比对。结果共获得37Mbp数据,平均读长为322bp。数据组装后,得到170个序列重叠群,总长为3.96Mbp。与参考序列相比,测定序列对于参考序列的覆盖度达到99.49%,参考序列96.76%的基因被完整测出同源基因。结论 454 GS Junior测序平台可以用于细菌基因组的快速测定。
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| 关 键 词： | 454 GS Junior测序平台 细菌基因组测序 |
Evaluation of 454 GS Junior Sequencing Platform Using in Bacterial Genome de novo Sequencing |
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| CHEN Chen＊ ZHANG Yuan-yuan,WANG Hai-yin. Evaluation of 454 GS Junior Sequencing Platform Using in Bacterial Genome de novo Sequencing[J]. Guide of China Medicine, 2013, 0(14): 1-2 |
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| Authors: | CHEN Chen＊ ZHANG Yuan-yuan WANG Hai-yin |
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| Affiliation: | (State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China) |
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| Abstract: | Objective To investigate the application value of 454 GS Junior Sequencing Platform in bacterial genorne sequencing. Methods Genorne sequencing of Vibrio Cholera was performed using 454 pyrosequencing, and the genorne was compared with the reference sequence of N16961 after assembling and annotation. Results Totally, 37 Mbp raw data were obtained and the average reads length is 322 bps. 170 contigs were assembled and the whole genorne is 3.96 Mbp. Comparing with strain N16961, the sequenced genome covered 99.49% of the reference genorne and 96.76% genes of N16961 got orthologous in the sequenced genome. Conclusion The 454 GS Junior Sequencing Platform is suitable for the fast sequencing of bacterial genome. |
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| Keywords: | 454 GS Junior Sequencing Platform Bacterial Genome de novo Sequencing |
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