| 高效液相色谱法测定月季花中金丝桃苷含量 |
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| 引用本文： | 崔慧芳,常欣. 高效液相色谱法测定月季花中金丝桃苷含量[J]. 中国药业, 2013, 22(11): 4-5 |
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| 作者姓名： | 崔慧芳 常欣 |
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| 作者单位： | 崔慧芳 (浙江省宁波市药品检验所,浙江宁波,315048)； 常欣 (浙江省宁波市药品检验所,浙江宁波,315048)； |
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| 摘 要： | 目的建立测定月季花中金丝桃苷含量的高效液相色谱法。方法色谱柱为Agilent C18柱(100 mm×4.6 mm,2.7μm),流动相为乙腈-0.05%磷酸溶液(16∶84),检测波长为360 nm,流速为0.5 mL/min。结果金丝桃苷进样量在0.047 68～0.476 80μg范围内与峰面积呈良好线性关系,平均回收率为97.22%,RSD=0.89%(n=9)。结论该方法快速简便,稳定可靠,专属性强。
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| 关 键 词： | 高效液相色谱法 月季花 金丝桃苷 含量测定 |
Determination of Hyperin Content in Rosa chinensisJacq by HPLC |
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| Cui Huifang,Chang Xin. Determination of Hyperin Content in Rosa chinensisJacq by HPLC[J]. China Pharmaceuticals, 2013, 22(11): 4-5 |
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| Authors: | Cui Huifang Chang Xin |
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| Affiliation: | (Ningbo Municipal Institute for Drug Control,Ningbo,Zhejiang,China 315048) |
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| Abstract: | Objective To establish a HPLC method for determining the hyperin content in Rosa chinensis Jacq.Methods The Agilent C18 column（100 mm×4.6 mm,2.7 μm） was adopted with the mobile of acetonitrile-0.05%phosphoric acid（16 ∶84）;the detection wavelength was set at 360 nm with flow rate of 0.5 mL/min.Results A good linearity relation was observed between the sample size of hyperin during the range of 0.047 68-0.476 80 μg with the peak area.The average recovery rate of hyperin was 97.22%,RSD=0.89%（n=9）.Conclusion This method is rapid,convenient,stable,reliable and strongly specific for the dermination of hyperin in Rosa chinensis Jacq. |
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| Keywords: | HPLC rosa chinensis Jacq hyperin content determination |
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