| 中药复方君子汤联合环孢霉素A逆转白血病细胞諯562/VCR耐药性的实验研究 |
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| 引用本文： | 廖斌,葛仁英,陈霞,皇甫真萍,齐彦,宋永平,魏旭东. 中药复方君子汤联合环孢霉素A逆转白血病细胞諯562/VCR耐药性的实验研究[J]. 中国实验血液学杂志, 2007, 15(4): 752-755 |
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| 作者姓名： | 廖斌 葛仁英 陈霞 皇甫真萍 齐彦 宋永平 魏旭东 |
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| 作者单位： | 1. 福建省人民医院、福建中医学院附属人民医院血液科,福州,350004
2. 河南省肿瘤医院血液科、河南省血液病研究所,郑州,450003 |
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| 基金项目： | 福建省教育厅A类科研课题基金 |
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| 摘 要： | 本研究观察中药复方君子汤(FFJZ)联合环孢霉素A(cyclosporine A,CsA)逆转白血病耐药细胞系K562/VCR细胞耐药性的效果,以便寻找有效的联合逆转剂.采用MTT法、流式细胞术研究FFJZ联合CsA逆转白血病耐药细胞系K562/VCR耐药性的作用.结果表明FFJZ联合CsA在体外对K562/VCR细胞的耐药性有明显的逆转作用(p＜0.01),能提高K562/VCR细胞对阿霉素(ADM)的敏感性,且在药物有效浓度范围内对细胞本身无毒性作用.FFJZ联合CsA对K562/VCR细胞的P-gp表达的阳性率无明显影响.结论复方君子汤联合环孢霉素A可能成为治疗白血病多药耐药的安全有效的耐药逆转药物.
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| 关 键 词： | 白血病 多药耐药 中药复方君子汤 环孢霉素A K562/VCR细胞 P-gp蛋白 |
| 文章编号： | 1009-2137(2007)04-0752-04 |
| 修稿时间： | 2006-08-12 |
Synergistic Reversal Effect of Chinese Medicine Compound FFJZ Combined with Cyclosporine A on Multidrug Resistance of Leukemia K562/VCR Cell Line |
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| LIAO Bin,GE Ren-Ying,CHEN Xia,HUANGFU Zhen-Ping,QI Yan,SONG Yong-Ping,WEI Xu-Dong. Synergistic Reversal Effect of Chinese Medicine Compound FFJZ Combined with Cyclosporine A on Multidrug Resistance of Leukemia K562/VCR Cell Line[J]. Journal of experimental hematology, 2007, 15(4): 752-755 |
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| Authors: | LIAO Bin GE Ren-Ying CHEN Xia HUANGFU Zhen-Ping QI Yan SONG Yong-Ping WEI Xu-Dong |
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| Affiliation: | 1.Department of Hematology, The People Hospital of Fujian Province, Fufian Traditional Chinese Medical College, Fuzhou 350004, China ; 2.Department of Hematology, Tumor Hospital of Henan Province, Henan Institiute of Hematology, Zhengzhou 450003, China |
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| Abstract: | The study was purposed to investigate the synergistic reversal effect of Chinese medicine compound FFJZ in combination with cyclosporine A(CsA) on the multidrug resistance (MDR) of human leukemia K562/VCR cell line,as to search effective combination of MDR modulators. MTT (methyl-thazol-tetrazolinum) assay were used to determine the cytotoic and reversal effects on K562/VCR cell line, FCM (flow cytometry) was used to assess the intracellular adriamycin (ADM) concentration and the expression of P-gp in cells. The rasults showed that the FFJZ in combination with CsA could reverse the drug-resistance of K562/VCR cells and increase the sensitivity K562/VCR cells to adriamycin. They had not the toxic effect on the K562/VCR cells in effective dose and no significant influence on P-gp positive rate of the K562/VCR cells. It is concluded that the FFJZ in combination with CsA may become a safe and effective multidrug resistance-reversing agent with low toxicity in leukemia chemotherapy. |
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| Keywords: | leukemia multidrug resistance Chinese medicine compound FFJZ cyclosporine A K562/VCR cell Pglycoprotein |
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