| 标本保存温度和保存时间对流式自然杀伤细胞和B细胞数量检测的影响 |
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| 引用本文： | 周方圆,张蕴莉. 标本保存温度和保存时间对流式自然杀伤细胞和B细胞数量检测的影响[J]. 军医进修学院学报, 2014, 0(3): 283-285 |
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| 作者姓名： | 周方圆 张蕴莉 |
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| 作者单位： | 辽宁医学院附属第一医院 检验科,辽宁锦州121000 |
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| 摘 要： | 目的：检测标本保存时间和保存温度，为流式自然杀伤（natural killer，NK）细胞和B细胞检测标准化操作提供科学依据。方法采用四色流式细胞术分析健康体检人群外周血NK细胞和B细胞数量百分比，检测保存时间与保存温度对实验结果的影响。结果未经处理的全血标本常温（18℃）保存后检测结果稳定，低温、高温及长时间保存都引起检测结果降低；全血标本经荧光抗体染色后，用多聚甲醛固定可在4℃条件下保存1周。结论流式细胞分析法检测NK细胞和B细胞，若标本不能及时处理宜18℃保存并在96 h内检测；处理后的样本可在4℃条件下保存1周。
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| 关 键 词： | 自然杀伤细胞 B淋巴细胞 流式细胞术 |
Effect of sample storage temperature and time on NK cells and B-lymphocytes detected by flow cytometry |
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| ZHOU Fang-yuan,ZHANG Yun-li. Effect of sample storage temperature and time on NK cells and B-lymphocytes detected by flow cytometry[J]. Academic Journal of Pla Postgraduate Medical School, 2014, 0(3): 283-285 |
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| Authors: | ZHOU Fang-yuan ZHANG Yun-li |
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| Affiliation: | ( Clinical Laboratory, First Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China) |
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| Abstract: | Objective To study the effect of storage temperature and time on the detection of NK cells and B-lymphocytes. Methods NK cells and B-lymphocytes in peripheral blood were detected by 6-color flow cytometry. The effect of storage temperature and time on the detection of NK cells and B-lymphocytes was observed. Results No change was found in the number of NK cells and B-lymphocytes in whole blood samples stored at 18℃. The number of NK cells and B-lymphocytes decreased after stored at low or high temperature. The whole blood samples that were fixed with paraformaldehyde and stained with fluorescent antibody could be stored for 7 days. Conclusion Flow cytometry can detect NK cells and B-lymphocytes. The samples can be stored at 18℃for 96 h when it is not able to treat them in time. The treated samples can be stored at 4℃for 7 days. |
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| Keywords: | natural killer cell B-lymphocyte flow cytometry |
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