病理性瘢痕中脂质过氧化产物和铜锌超氧化物歧化酶的变化

目的了解病理性瘢痕（增生性瘢痕与瘢痕疙瘩）中脂质过氧化产物和铜锌超氧化物歧化酶（copper，zinc-superoxide dismutase，CuZn-SOD）的变化。方法取2005年5月-2005年8月收治患者自愿捐献的标本。瘢痕疙瘩组10例，年龄16～35岁，平均病程2．2年；增生性瘢痕组10例，年龄17～32岁，平均病程8个月；正常皮肤组8例，年龄16～34岁。应用化学比色法测定3组标本中CuZn．SOD活力和丙二醛（malonaldehyde，MDA）含量，采用免疫组织化学方法观察CuZn-SOD蛋白在病理性瘢痕中的表达，并对其进行评分。结果正常皮肤组、增生性瘢痕组及瘢痕疙瘩组MDA含量分别为（0．8213±0．0864）、（1．1390±0．1067）、（1．1900±0．0748）nmol／mg prot；CuZn-SOD活力分别为（20．60±5．56）、（31．65±2．21）、（34．36±5．01）U／mg prot。增生性瘢痕组和瘢痕疙瘩组MDA含量与CuZn-SOD活力同正常皮肤组比较，差异均有统计学意义（P〈0．05）；增生性瘢痕组及瘢痕疙瘩组间比较，差异无统计学意义（P〉0．05）。免疫组织化学染色观察：3组表皮角质形成细胞和真皮成纤维细胞均有CuZn．SOD蛋白阳性表达。正常皮肤组、增生性瘢痕组及瘢痕疙瘩组在表皮角质形成细胞中免疫组织化学评分分别为（2．20±0．45）、（4．14±0．90）、（4．43±0．79）分；在真皮成纤维细胞中评分分别为（1．60±0．89）、（4．00±0．82）、（4．43±0．53）分。增生性瘢痕组和瘢痕疙瘩组CuZn-SOD蛋白表达与正常皮肤组比较，差异有统计学意义（P〈0．05）；增生性瘢痕组及瘢痕疙瘩组间比较，差异无统计学意义（P〉0．05）。结论病理性瘢痕中脂质过氧化产物MDA含量增加，CuZn-SOD活力升高、表达增强。

VARIATIONS OF LIPID PEROXIDATION PRODUCTS AND COPPER, ZINC-SUPEROXIDE DISMUTASE IN PATHOLOGICAL SCARS

OBJECTIVE: To study the variations of lipid peroxidation products and copper, zinc-superoxide dismutase (CuZn-SOD) in pathological scars (hypertrophic scars and keloids). METHODS: The specimens were gained from patients of voluntary contributions from May 2005 to August 2005. The tissues of hypertrophic scar (10 cases, aged 16-35 years, the mean course of disease was 2.2 years), keloid (10 cases, aged 17-32 years, the mean course of disease was 8 months) and normal skin (8 cases, aged 16-34 years) were obtained. The content of malonaldehyde (MDA)and CuZn-SOD activity were detected by spectrophotometric method. The expression of CuZn-SOD was evaluated by immunohistochemistry technique. RESULTS: The contents of MDA and CuZn-SOD activity were significantly higher in hypertrophic scars MDA (1.1390 +/- 0.1067) nmoL/mg prot, CuZn-SOD (31.65 +/- 2.21)U/mg prot, (P < 0.05)] and keloids MDA (1.1900 +/- 0.074 8) nmoL/mg prot, CuZn-SOD (34.36 +/- 5.01) U/mg prot (P < 0.05)] than those of normal skin tissues MDA (0.8213 +/- 0.0864) nmoL/mg prot, CuZn-SOD (20.60 +/- 5.56) U/mg prot]. Immunohistochemical studies indicated that the brown particles were CuZn-SOD positive signals, which mainly located cytoplasm in normal skin tissues, hypertrophic scars as well as keloids epidermal keratinocytes and dermal fibroblasts. CuZn-SOD expression evaluation in hypertrophic scars (4.14 +/- 0.90, P < 0.05) and keloids epidermal keratinocytes (4.43 +/- 0.79, P < 0.05) markedly increased when compared with normal skin tissues (2.20 +/- 0.45). The expression of CuZn-SOD in hypertrophic scars (4.00 +/- 0.82, P < 0.05) and keloids dermal fibroblasts (4.43 +/- 0.53, P < 0.05) were significantly higher than that of normal skin tissues (1.60 +/- 0.89). There were no differences in the content of MDA, CuZn-SOD activity and expression evaluation between hypertrophic scars and keloids (P > 0.05). CONCLUSION: In pathological scars, the contents of MDA and CuZn-SOD activity increase and the expressions of CuZn-SOD are enlarged.