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p53 mutations and codon 213 polymorphism of p53 in lung cancers of former uranium miners |
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| Authors: | Walter Popp Carola Vahrenholz Hans Schuster Bernhard Wiesner Peter Bauer Frank Täuscher Helmut Plogmann Konrad Morgenroth Nikolaus Konietzko Klaus Norpoth |
| Affiliation: | Institute of Hygiene and Occupational Medicine, University of Essen, Hufelandstr. 55, D-45122 Essen, Germany Tel.: +49 201 7234574; Fax: +49 201 7234546, DE Surgery Clinic 2, Medical Center of Gera, Stra?e des Friedens 122, D-07548 Gera, Germany, DE Central Clinics, D-99438 Bad Berka, Germany, DE Department of Pneumoloy (Ruhrlandklinik), University of Essen, Tüschener Weg 40, D-45239 Essen, Germany, DE Clinic for Occupational Diseases, Lauterbacher Str. 16, 08223 Falkenstein, Institute of Pathology, Ruhr-University, D-44780 Bochum, Germany, DE |
| Abstract: | Purpose: There is a high prevalence of G→T transversions of p53 in lung cancers of smokers. One study has reported a special “hotspot” mutation at codon 249 of p53 in lung cancers of former uranium miners. The aim of our study was to look for mutational spectra of p53 in former German uranium miners with lung cancers. Methods: We investigated 16 patients with lung cancer who had worked as uranium miners in Germany and 13 lung cancer patients without a mining history of the same region. By means of the polymerase chain reaction and sequencing we looked for mutations in exons 5–7 of the p53 gene. Results: We could not find any suggestion of hotspot mutations. The only G→T mutation in former uranium miners was detected in the only nonsmoker. In 3 patients (19% of the total) we found a codon 213/3 polymorphism. Conclusions: The results indicate that G → T transversions do not seem to be very common mutations in p53 in lung cancers probably caused by radiation. Therefore, p53 may be mutated early in lung cancer development if radiation exposure is a critical factor in carcinogenesis. In accordance with studies of thyroid cancer patients in the Chernobyl region, our results may indicate an overrepresentation of codon 213/3 polymorphism in p53 in radiation-caused cancers. Received: 24 September 1998 / Accepted: 16 December 1999 |
| Keywords: | Lung cancer p53 Codon 213 polymorphism Radon Uranium mining |
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