前列腺癌PC-3M细胞hmSD活性与细胞凋亡相关性的实验研究

目的：研究前列腺癌PC-3M细胞株hmSD的活性与细胞凋亡的相关性。方法： 应用丁酸钠诱导前列腺癌PC-3M细胞发生凋亡，吖啶橙/溴化乙锭染色检测细胞凋亡率；收集丁酸钠作用后的PC-3M细胞，匀浆后离心取上清，与底物共同孵育，以硫代巴比妥酸（TBA）显色反应检测所生成的唾液酸，计算唾液酸酶活性；将细胞凋亡率与hmSD活性进行相关性分析。结果：丁酸钠诱导PC-3M细胞凋亡的同时显著下调hmSD的活性，凋亡率与hmSD活性呈负相关。结论：hmSD活性变化与前列腺癌细胞凋亡呈相关性。

Relation between hmSD activity and apoptosis rate in a PC-3M cell line

AIM: To investigate the correlation betwe en human membrane-associated sialidase (hmSD) activity and apoptosis rate in a PC- 3M cell line. METHODS: Sodium butyrate was added to the culture media of PC-3M cells for 48 h to induce apoptosis and then the cells were collected, homogenat e d and centrifuged. The supernatant was incubated with the substrate. TBA reactio n was then used to detect the sialic acid and the activity of the sialidase was calculated. Meanwhile, the apoptosis rate was detected by AO/EB assay, and the c orrelation between hmSD activity and apoptosis rate was calculated. RESULTS: HmSD activity of PC-3M cells was significantly down-reg ulated when cell apoptosis was induced by sodium butyrate and high correlation b etween hmSD activity and apoptosis rate was observed. CONCLUSION: There is a significant correlation between apoptosis rate and hmSD activity in prostate cancer.