| 溶酶体组织蛋白酶 L在小鼠病毒性心肌炎心肌组织中表达及意义 |
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| 引用本文： | 杜九中,于小华,吕建华,邓晖,李双杰,赵刚,张新刚,王时俊,陈瑞珍,杨英珍.溶酶体组织蛋白酶 L在小鼠病毒性心肌炎心肌组织中表达及意义[J].临床儿科杂志,2005,23(6):388-390. |
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| 作者姓名： | 杜九中 于小华 吕建华 邓晖 李双杰 赵刚 张新刚 王时俊 陈瑞珍 杨英珍 |
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| 作者单位： | 南华大学附属第一医院儿科,湖南衡阳,421001;复旦大学附属中山医院心研所 |
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| 基金项目： | 国家自然科学基金资助课题(编号:30271665) |
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| 摘 要： | 目的观察Balb/c小鼠病毒性心肌炎心肌组织组织蛋白酶L(cathepsinL)的表达及意义。方法CVB3m(Nancy株)病毒感染Balb/c小鼠建立病毒性心肌炎实验模型,采用免疫组化和RT-PCR技术,检测小鼠心肌组织中不同时点(正常和感染后第3、7、15、30天)cathepsinL的表达。结果免疫组化与RT-PCR结果显示,感染CVB3m后小鼠心肌组织中cathepsinL的表达随感染时间的延长,呈上升趋势,至30d表达最高,各时点cathepsinL的表达水平与正常小鼠比较差异有显著性(P<0.05)。结论cathepsinL在Balb/c小鼠CVB3病毒性心肌炎的发生发展起一定作用。
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| 关 键 词： | 溶酶体 组织蛋白酶L 病毒性心肌炎 小鼠 |
| 文章编号： | 1000-3606(2005)06-388-03 |
| 修稿时间： | 2004年10月27 |
The expression and significance of lysosome cathepsin L in the cardiac tissue of viral myocarditis in mice |
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| Du Jiuzhong,Yu Xiaohua,Lu Jianhua,Deng Hui,Li Shuangjie,Zhao Gang,Zhang Xingang,Wang Shijun,Chen Ruizhen,YANG Yingzhen.The expression and significance of lysosome cathepsin L in the cardiac tissue of viral myocarditis in mice[J].The Journal of Clinical Pediatrics,2005,23(6):388-390. |
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| Authors: | Du Jiuzhong Yu Xiaohua Lu Jianhua Deng Hui Li Shuangjie Zhao Gang Zhang Xingang Wang Shijun Chen Ruizhen YANG Yingzhen |
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| Institution: | Du Jiuzhong,Yu Xiaohua,Lu Jianhua,Deng Hui,Li Shuangjie,Zhao Gang,Zhang Xingang,Wang Shijun,Chen Ruizhen,Yang Yingzhen.Pediatric Department,the First Affiliated Hospital of Nanhua University,Hengyang,Hunan421001 |
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| Abstract: | Object To study the expression and significance of lysosome cathepsin L in the cardiac muscle of Balb/c mice infected by Coxsackie virus B(CVB 3 )(Nancy strain).Methods The model of Balb/c mice with CVB 3 (Nancy strain)infection was established.The expression of cathepsin L in the cardiac tissue was determined with immuohistochemical staining and RT-PCR at different time point(before infection and3,7,15,30days after infection respectively).Results In comparison with the control group,the expression of cathepsin L in the cardiac tissue of Balb/c mice with CVB 3 (Nancy strain)increased after infection with increasing tendency and the highest at30rd day.The difference of cathepsin L expression between normal group and the study group at every time point had statistic significance(P<0.05).Conclusions Cathepsin L might play certain role in the pathogenesis of CVB 3 myocarditis in Balb/c mice. |
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| Keywords: | lysosome cathepsin L viral myocarditis mice |
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