骨巨细胞瘤中成纤维样基质细胞体外钙化能力的研究

目的：探讨骨巨细胞瘤的组织来源。方法：体外培养。Ｇｏｍｏｒｉ法碱性磷酸酶染色，生化检测细胞培养上清液中的碱性磷酸酶含量。免疫组织化学染色检测骨钙素在成纤维样基质细胞中的表达。使用含Ｂ甘油磷酸钠和ＣａＣｌ２的培养液，观察成纤维样基质细胞体外钙化能力。结果：成纤维样基质细胞中碱性磷酸酶和骨钙素呈阳性表达，在体外具有钙化能力。在细胞培养上清液中有碱性磷酸酶。结论：骨巨细胞瘤中的成纤维样基质细胞具有某些成骨细胞的特点，为进一步探讨其组织来源提供了实验依据。

Calcification by the fibroblast-like stromal cell from giant cell tumour of bone in vitro

Objective: To study the origin of the giant cell tumour of bone(GCT). Methods: It was culture in vitro . The expression of alkaline phosphatase (AlP) in the fibroblast like stromal cell (FSTC) was detected by Gomori method and the osteocalcin expression by immunhistochemistry method. AlP in the culture medium was assayed by biochemical method. The capability of calcification in vitro was by FSTC. Results: FSTC expressed AlP and osteocalcin. In the culture medium, there was AlP also. FSTC could format calcification grain in vitro . Conclusion: FSTC has some osteoblast features. Our findings may provide a basis for further discussing the origin of the GCT.