| 钉螺一氧化氮合酶分布的酶组织化学研究 |
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| 引用本文： | 梁幼生,戴建荣,朱荫昌,周晓农,张艳,熊希凯,黄轶昕,杭盘宇. 钉螺一氧化氮合酶分布的酶组织化学研究[J]. 中国血吸虫病防治杂志, 2001, 13(4): 196-197,255 |
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| 作者姓名： | 梁幼生 戴建荣 朱荫昌 周晓农 张艳 熊希凯 黄轶昕 杭盘宇 |
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| 作者单位： | 1. 江苏省血吸虫病防治研究所
2. 华中科技大学同济医学院解剖学教研室 |
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| 基金项目： | 本研究为国家自然科学基金资助课题(批准号:30070684)内容之一. |
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| 摘 要： | 目的 定位研究一氧化氮合酶（NOS）在日本血吸虫中间宿主－钉螺体内分布及活性。方法 用还原型尼可酰胺腺嘌呤二核苷酸磷酸硫辛酰胺脱氢酶（NADPH－d)酶组织化学技术，对钉螺软体的整体连续切片作系统观察。结果 头足部肌纤维间含丰富NOS阳性神经元；中枢神经节纤维区、心脏壁、雌雄生殖细胞、咽管等呈NOS的强阳性；腮管壁呈NOS阳性。结论 NO作为重要生物信使，直接参与钉螺神经肌肉运动、生殖和心血管调节等多种生命活动。
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| 关 键 词： | 钉螺 一氧化氮合酶 NADPH－d 酶组织化学 灭钉螺 日本血吸虫 |
| 文章编号： | 1005-6661(2001)04-0196-02 |
AN ENZYME-HISTOCHEMICAL STUDY OF DISTRIBUTION OF THE NITRIC OXIDE SYNTHASE IN ONCOMELANIA HUPENSIS |
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| Liang Yousheng ,Dai Jiangrong ,Zhu Yinchang ,Zhou Xiaonong ,Zhang Yan ,Xiong Xikai ,Huang Yixin ,Hang Panyu Jiangshu Institute of Parasitic Diseases Wuxi . AN ENZYME-HISTOCHEMICAL STUDY OF DISTRIBUTION OF THE NITRIC OXIDE SYNTHASE IN ONCOMELANIA HUPENSIS[J]. Chinese journal of schistosomiasis control, 2001, 13(4): 196-197,255 |
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| Authors: | Liang Yousheng Dai Jiangrong Zhu Yinchang Zhou Xiaonong Zhang Yan Xiong Xikai Huang Yixin Hang Panyu Jiangshu Institute of Parasitic Diseases Wuxi |
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| Affiliation: | Liang Yousheng 1,Dai Jiangrong 1,Zhu Yinchang 1,Zhou Xiaonong 1,Zhang Yan 2,Xiong Xikai 2,Huang Yixin 1,Hang Panyu 1 1 Jiangshu Institute of Parasitic Diseases Wuxi 214064, 2 Department of Anatomy,Tongji School of Medicine,Huazhong Scien |
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| Abstract: | Objective To study the distribution and activity of nitric oxide synthase in the tissues of the schistosome vector snail, Oncomelania hupensis.Methods By way of nicotinamide adenosine dinucleotide phosphate diaphorase (NADPH d) histochemical technique the continued sections of Oncomelania hupensis snails were investigated systematically. Results The richest NOS positive neurons were found among muscle cells in the head foot region. The strong NOS positive reactions were demonstrated respectively in the center ganglions,heart wall,testes,ovary,pharyngeal cavity and ctenidium wall of the snails. Conclusion Nitric oxide, as a important biological messenger, is involved in regulating the movement of both nerves and muscles, the functions of reproduction and the cardiac system directly in Oncomelania hupensis. |
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| Keywords: | Oncomelania hupensis Nitric oxide synthase NADPH d Enzyme histochemistry |
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