梅毒螺旋体特异性ASC诊断梅毒螺旋体活动性感染研究

目的建立一种酶联免疫法来检测病人体内的特异性ASC。据此判断其体内是否有活动性梅毒螺旋体感染。方法采用梅毒重组融合抗原ELISA试剂盒分别检测正常人群、潜伏期梅毒、一期梅毒、二期梅毒患者的特异性ASC。结果各病例组均检出特异性ASC，一期梅毒组的OD均值明显高于二期梅毒组和潜伏梅毒组，差异具有显著性（P〈0．01）。GST—epi—TpNl7抗原检测灵敏度优于GST—TpNl7融合抗原。结论本研究建立的梅毒螺旋体特异性ASC检测方法可用于活动性梅毒感染的诊断以及作为疗效观察的指标，为临床检测梅毒感染提供了新思路。

Use of specific circulating antibody-secreting cells（ASC） for the diagnosis of active Treponema pallidum infection

Objective： Developing a recombinant fusion antigen-based ELISA to diagnose actwe I reponema pallidum infection by detecting TP-specific ASC. Methods： Specific ASC from group of primary syphilis, secondary syphilis and latent syphilis has been detected using recombinant fusion antigen-based ELISA kits, healthy people as control. Results： Specific ASC were demonstrated in all syphilis group, average OD of primary syphilis was significantly higher than the other two groups（P〈0.01）. ELISA equipped with the GST-epi-TpN17 antigen showed higher sensitivity as compared with GST -TpN17＇s. Conclusion： The recombinant fusion antigen-based ELISA was suitable for the diagnosis of active TP infection and monitoring efficacy of antibiotic therapy.