梅毒胶体金法在临床诊断中的应用价值

目的了解梅毒胶体金法在自愿咨询检测(VCT)门诊、社区卫生服务中心和非政府组织(NGO)工作室等临床诊断中的应用价值。方法应用梅毒螺旋体(TP)抗体检测试剂(胶体金法)、酶联免疫吸附试验(ELISA)、梅毒螺旋体明胶颗粒凝集试验(TPPA),同时对2011-2013年医院哨点监测性病门诊送检的血清标本进行检测。结果对1204例样本进行检测,梅毒胶体金法阳性检出率为20.51%(247/1204),ELISA法为21.01%(253/1204),TPPA法为20.02%(241/1204)。以TPPA为金标准,梅毒胶体金、ELISA的敏感性均为99.59%,特异性分别为99.27%、98.65%。胶体金法与TPPA法和ELISA法经Kappa一致性检验比较(u分别为135.20、137.71,k=0.98,P0.05),具有极强的一致性。结论梅毒胶体金检测敏感性高、特异性强,具有简便、迅速的特点,值得在临床诊断中推广应用。

Application value of colloidal gold method in clinical diagnosis of syphilis

Objective To investigate application value of colloidal gold method in clinical diagnosis of syphilis in volun-tary counseling testing （VCT）, community health service and non-governmental organizations （NGOs） studio. Conclusion Colloidal gold method, enzymelinked immunosorhent assay （ELISA） and treponema pallidum gelatin agglutination test （TPPA） were used at the same time for serum samples in clinics of STD sentinel hospitals between 2011 and 2013. Results Syphilis Detection rate by colloidal gold method was 20. 51 % （247/1204）, 21.01 %（253/1204） by ELISA, and 20. 02 % （241/1204） by TPPA. Taking TPPA as the gold standard, the sensitivity of both syphilis colloidal gold and ELISA was 99. 59 %, while the specificity was 99.27 % and 98. 65 0%, respectively. Colloidal gold method had strong consistency with TPPA method and ELISA method using Kappa consistency test （u was 135.20 and 135.20, respectively, k= 137. 71, P〈0. 05）. Conclusion Colloidal gold syphilis detection is simple and rapid has with high sensitivity,and strong specificity, and is worth of popularization and application in clinical diagnosis.