| 中波紫外线对人角质形成细胞iNOS的作用 |
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| 引用本文： | 杨敏,王宝玺,常建民,左亚刚,闫言,渠涛,乔菊.中波紫外线对人角质形成细胞iNOS的作用[J].中国皮肤性病学杂志,2005,19(7):401-403. |
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| 作者姓名： | 杨敏 王宝玺 常建民 左亚刚 闫言 渠涛 乔菊 |
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| 作者单位： | 1. 中国医学科学院中国协和医科大学北京协和医院皮肤科,北京,100730
2. 卫生部北京医院皮肤科,北京,100730 |
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| 基金项目： | 中华医学会-欧莱雅中国人健康皮肤研究项目(2004010525) |
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| 摘 要： | 目的观察中波紫外线(UVB)照射对体外培养正常人角质形成细胞(HKC)中诱导型一氧化氮合成酶(iNOS)的作用,探讨紫外线引起皮肤细胞急性损伤的发病机制。方法体外培养HKC,用免疫荧光染色和蛋白印迹技术检测照射后不同时间细胞内iNOS的水平。结果HKC经UVB照射后,免疫荧光法检测到细胞膜胞浆侧有iNOS,蛋白印迹法检测到细胞提取总蛋白中分子量为130kDa的蛋白条带可以被抗iNOS多克隆抗体所识别,二者结果相符合。结论UVB照射可诱导体外培养的HKC在胞浆内产生iNOS,iNOS蛋白的表达可能与紫外线引起的皮肤急性炎症反应有关。
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| 关 键 词： | 中波紫外线 人角质形成细胞 诱导型一氧化氮合酶 |
| 文章编号： | 1001-7089(2005)07-0401-03 |
Level of iNOS Synthase in Cultured Human Keratinocytes effected by UVB Irradiation |
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| YANG Min,WANG Bao-xi,CHANG Jian-min,et al.Level of iNOS Synthase in Cultured Human Keratinocytes effected by UVB Irradiation[J].The Chinese Journal of Dermatovenereology,2005,19(7):401-403. |
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| Authors: | YANG Min WANG Bao-xi CHANG Jian-min |
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| Abstract: | Objective To observe the level of inducible nitric oxide (iNOS) synthase in cultured normal human keratinocytes after UVB irradiation and study the pathogenesis of UV induced acute damage of the skin.Methods Normal human keratinocytes were cultured and the cells were irradiated with UVB at different dosage.We detected the level of iNOS by immunofluorescent staining and Western-blotting after UVB irradiation.Results The level of iNOS protein in UVB irradiated cultured keratinocytes was identified by immunofluorescent staining,which was more prominent at the inner side of cell membrane. Western-blot also revealed a 130 kDa protein reacted with polyclonal anti-iNOS antibodies.Conclusion UVB irradiation induced iNOS synthase which may take part in the pathogenesis of UV related acute inflammation of the skin. |
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| Keywords: | UVB Human keratinocytes iNOS |
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