感觉神经(元)对失神经骨骼肌超微结构保护作用的实验研究

目的观察感觉神经（元）对失神经骨骼肌超微结构的保护作用。方法60只SD大鼠,按手术先后顺序随机分成10组，每组6只。A组(对照组)肱二头肌完全失神经支配。B～E组(实验组)B组为失神经支配加感觉神经种植组，C组为失神经支配加感觉神经寄养组，D组为失神经支配加背根神经节种植组，E组为节前撕脱的感觉神经种植组。术后1、3个月取材，各组又分为二个时间组。用透射电镜观察肌肉超微结构的变化。结果与对照组相比，各实验组肌肉退变核数少、线粒体肿胀及肌质网扩张程度轻、毛细血管/肌纤维数比值大、间质胶原纤维和成纤维细胞少、肌丝肌节排列整齐。结论感觉神经（元）对失神经骨骼肌超微结构有保护作用。

Experimental study of the influence of sensory nerve (neuron) protection on the ultrastructure of denervated skeletal muscles

Objective To observe the influence of sensory nerve (neuron)protection on the ultrastructure of denervated skeletal muscle. Methods 60 SD rats were randomly divided into 10 groups, with 6 each. Group A of simple complete denervation of biceps brachii served as control. Groups B ～ E were experimental groups which standed for complete denervation with sensory nerve implantation, complete denervation with sensory nerve “ baby - sitting”, complete denervation with dorsal root ganglia implantation, and complete denervation with implantation of preganglionically avulsed sensory nerve respectively. 1 month and 3 months postoperatively, electron microscopic observation of the muscles was done. Results Compared to the control, less degenerated nucleus, minor mitochondria edema and sarcoplasmic reticulum enlargement, higher capillary/myofiber ratio, less interstitial fibroblast and collagen fibers, better oriented myomere and myofilament were seen in the experimental groups. Conclusions Sensory nerves or neurons can protect the ultrastructure of denervated skeletal muscle.