健脾益气法治疗严重软组织损伤的实验研究

目的：观察健脾益气法对严重创伤大鼠软组织修复过程中新生毛细血管和成纤维细胞的影响,进一步探讨依据“脾主四肢、肌肉”理论治疗严重创伤软组织损伤的作用机制。方法：采用股动脉放血结合刀割法制备严重创伤大鼠模型,然后将实验大鼠随机分为模型组、健脾组、活血化瘀组。在治疗后第5、10、15天于创面中心区取材,采用组织固定切片后行CD31和PCNA染色,运用多功能显微镜（ZEISS Axioskop2）观察肉芽组织中新生毛细血管和成纤维细胞,用Image-ProPlus图像分析软件对肉芽组织中新生的毛细血管和成纤维细胞进行定量分析。结果：治疗后第5、10、15天健脾组毛细血管和成纤维细胞的增殖明显高于模型组（P〈0.05）,同时毛细血管和成纤维细胞的增殖速度和胶原化速度都比模型组和活血化瘀组快。结论：健脾益气法能有效促进严重创伤后肉芽组织的生长,加速伤口的愈合,且比传统疗法在创伤早期单纯应用活血化瘀药疗效更好。

Experimental study on the treatment of serious soft tissue injuries with strengthening the spleen and replenishing qi

Objective：To study the effects of Chinese drugs based on strengthening the spleen and replenishing qi treatment rule on neoformative capillaries and fibroblast during the soft tissue repair after serious trauma in rats,so as to explore the biological basis of the TCM theory＂the spleen dominate extremities and muscles＂ applied to the treatment of soft tissue injuries. Methods：The model rats were established by bleeding from femoral artery and lancing method, and the rats were randomly divided into the control group,strengthening the spleen group and activating blood and resolving stasis group. The samples were got from the tissue of the wounded area at the 5th, 10th and 15th days after oral administration of the traditional Chinese medicine. After fixation and section, the tissues were stained by CD31 and PCNA staining. The amount of the capillaries and fibroblasts in the tissue of the wounded area were observed through muhi-purpose microscope （ZEISS Axioskop2）. Quantitative analysis was carried out on Image-ProPlus image analyzer. Results ： The amount of the capillaries and fibroblasts in the wounded tissue in the strengthening the spleen group were larger than that in the control group at the 5th, 10th and 15th day. And the proliferation speed of capillaries and fibroblasts was faster than those in the control group or the activating blood and resolving stasis group. Conclusion ：The Chinese drugs according to strengthening the spleen and replenishing qi treatment rule were effective to promote growth of the granulation tissue and facilitate healing of the wounded area. And it has better effect than the treatment of promoting blood circulation and removing stasis.