蛭龙活血通瘀胶囊对心肌梗死大鼠血管新生的作用研究

目的：通过观察蛭龙活血通瘀胶囊对心肌梗死大鼠血管新生的影响，探讨蛭龙活血通瘀胶囊对心肌梗死大鼠模型心肌保护的可能机制。方法：采用冠状动脉左前降支动脉结扎法制备心肌梗死模型大鼠。健康SD大鼠60只，随机分为假手术组、模型组、蛭龙活血通瘀胶囊低、中、高剂量组、通心络组，每组大鼠10只。术后假手术组及模型组每日予生理盐水灌胃，其余各组分别给予相应药物灌胃14d。分别于术后7d、14d检测血清内皮生长因子（VEGF）；术后14d免疫组化检测梗死心肌边缘区新生血管数。结果：术后第7、14d，与假手术组比较，模型组、低、中、高剂量组及通心络组VEGF均升高，差异有统计学意义（P〈0．01）。各药物治疗组与模型组比较VEGF升高（P〈O．05），其中高剂量组及通心络组升高明显，差异有统计学意义（P〈0．01）。与通心络组比较，低剂量组、中剂量组差异有统计学意义（P〈O．05），高剂量组差异无统计学意义（P〉0．05）。MVC计数结果：与假手术组比较，模型组、低、中、高剂量组及通心络组均增加（P〈0．01），与模型组相比，各药物治疗组MVC均升高（P〈0．01）。与通心络组比较，低剂量组、中剂量组差异有统计学意义（P〈O．05），高剂量组差异无统计学意义（P〉O．05）。结论：蛭龙活血通瘀胶囊可促进心肌梗死边缘区新生血管形成，其机制可能与促进血清VEGF表达及维持VEGF高分泌状态有关。

Effect of Zhilong Huoxue Tongyu capsule on angiogenesis in rats with myocardial infarction

Objective： To study the effect of Zhilong Huoxue Tongyu capsule （ZHTC） on angiogenesis in myocardial infarction rats, its and its possible mechanism. Methods：The model of rat was prepared by ligating the left anterior descending branch of coronary artery. 60 healthy SD male rats were randomly divided into sham group, model group, low-dose, middle-dose, and high dose ZHTC groups,and Tongxinluo group, 10 rats in each group. In sham group and model group, rats were given saline ]avage daily;other groups were given corresponding drug lavage for 14 days after operation. At the 7th and 14th day, serum levels of vascular endothelial growth factor （VEGF） were detected,and the rats were killed,the number of neogenetic microvasular ceils （MVC） were detected by immunohistochemical method at the margin of infarction. Results： At the 7th and 14th day, compared with sham group, VEGF of the other 5 groups were higher, the difference was statistically significant （P 〈 0.01）. Compared with model group, VEGF of the 4 treatment groups were higher （P 〈 0.05）, of which the high dose group and Tongxinluo group were more significantly increased （P 〈 0.01 ）. Compared with Tongxinluo group, low dose and middle dose ZHTC groups was statistically significant （P 〈 0.05）, high dose ZHTC group had no statistically significant difference （P 〉 0.05）. MVC counting results：at the 14th day, however compared with sham group, the other 5 groups had significant changes （P 〈 0.01 ）; compared with model group, the MVC of treatment groups were higher （P 〈 0.01 ）. Compared with Tongxinluo group, difference of low dose and middle dose ZHTC groups was statistically significant （P 〈 0.05）, high dose group had no statistically significant difference （P 〉 0.05）. Gonclusion： Zhilong HuoxueTongyu capsule can promote angiogenesis form the margin of myocardial infarction, its mechanism may be associated with increasing serum levels of VEGF and maintaining high VEGF secretion state.