| 鱼藤酮对大鼠纹状体谷氨酸-谷氨酰胺环路的影响 |
| |
| 引用本文： | 刘辉,郭魁亮,吴强,董兆君.鱼藤酮对大鼠纹状体谷氨酸-谷氨酰胺环路的影响[J].劳动医学,2009(2):159-161. |
| |
| 作者姓名： | 刘辉 郭魁亮 吴强 董兆君 |
| |
| 作者单位： | 白求恩军医学院卫勤教研室;第三军医大学毒理学教研室; |
| |
| 基金项目： | 国家自然科学基金资助项目(编号:30400347) |
| |
| 摘 要： | 目的]观察鱼藤酮（rotenone）对大鼠纹状体谷氨酸一谷氨酰胺环路代谢的影响。方法]将24只SD大鼠随机分为正常对照组、溶剂对照组、2.0mg／kg和4.0mg／ks鱼藤酮染毒组。采用高效液相色谱法（HPLC）检测鱼藤酮染毒大鼠纹状体谷氨酸（glutamate，Glu）和谷氨酰胺（glutamine，Gin）含量；使用逆转录聚合酶链式反应（RT-PCR）法观察谷氨酰胺合成酶（glutamine synthetase，GS）基因表达的变化；采用分光光度法检测谷氨酰胺酶（phosphate activated glutaminase，PAG）与GS活性。结果]与正常对照组比较，4.0mg／kg染毒组大鼠纹状体Glu含量明显升高（P〈0.01），而Gin含量明显降低（P〈0.01）；4.0mg／kg染毒组GSmRNA表达明显增强（P〈0.05）；4.0mg／kg染毒组Gs活性却明显降低，PAG活性明显升高（P〈0.01）。结论]鱼藤酮诱导大鼠纹状体Glu含量明显升高，可能是导致中脑神经元兴奋性损伤的的主要原因之一。
|
| 关 键 词： | 鱼藤酮 谷氨酸 谷氨酰胺 谷氨酰胺合成酶 谷氨酰胺酶 |
Effects of Rotenone on Glutamate-glutamine Circulation in Rats Striatum |
| |
| LIU Hui,GUO Kui-liang,WU Qiang,DONG Zhao-jun.Effects of Rotenone on Glutamate-glutamine Circulation in Rats Striatum[J].Journal of Labour Medicine,2009(2):159-161. |
| |
| Authors: | LIU Hui GUO Kui-liang WU Qiang DONG Zhao-jun |
| |
| Institution: | 21.Department of Health Service;Norman Bethune Military Medical College;Shijiazhuang;Hebei 050081;China;2.Department of Toxicology;The Third Millitary Medical University;Chongqing 400038;China |
| |
| Abstract: | Objective] To study the effects of rotenone on glutamate-glutamine circulation in rats striatum.Methods] 24 Sprague-Dawley rats were divided into four groups randomly:the control group,solvent control group,2.0 mg/kg and 4.0 mg/kg rotenone treated groups.The concentration of glutamate and glutamine in rats striatum were assayed by high performance liquid chromatography(HPLC).The expression of glutamine synthetase(GS)mRNA was measured by RT-PCR,and the activities of GS and phosphate activated glutaminase(P... |
| |
| Keywords: | rotenone glutamate gluatamine glutamine synthetase phosphate activated glutaminase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
